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Coordination environment and fluoride binding of type 2 copper in the blue copper oxidase ceruloplasmin

Dawson, John H. and Dooley, David M. and Gray, Harry B. (1978) Coordination environment and fluoride binding of type 2 copper in the blue copper oxidase ceruloplasmin. Proceedings of the National Academy of Sciences of the United States of America, 75 (9). pp. 4078-4081. ISSN 0027-8424. PMCID PMC336053.

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The electron paramagnetic resonance (EPR) spectra of the blue copper oxidase ceruloplasmin [ferroxidase, iron(II):oxygen oxidoreductase, EC] and of a derivative having the type 1 (blue) copper centers reversibly bleached are reported. The EPR spectrum of bleached ceruloplasmin has a seven-line superhyperfine structure in the g[perpendicular] region that is attributed to the presence of three nitrogen-donor type 2 copper ligands. The EPR data suggest further that the type 2 copper in ceruloplasmin possesses a tetragonal coordination gometry. In the presence of varying amounts of fluoride, superhyperfine splitting patterns in the g[parallel] region of both ceruloplasmin derivatives indicate that a maximum of two fluorides may be bound to the type 2 copper.

Item Type:Article
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Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1978 by the National Academy of Sciences. Contributed by Harry B. Gray, June 5, 1978. We thank Prof. Maria Linder (California State University at Fullerton) for gel electrophoretic analysis and enzyme assays of purified protein samples, Dr. Lewis Larsen for supplying us with Cohn Fraction IV, and Dr. Lars Ryden for helpful discussions about the protein purification. This research was supported by National Science Foundation Grant CHE77-11389. J.H.D. gratefully acknowledges support from National Institutes of Health Postdoctoral Fellowship 1 F32CA05748-01 for 1976-1978. D.M.D. acknowledges a National Institutes of Health Predoctoral Traineeship (1974-1978). This is contribution 5801 from the Arthur Amos Noyes Laboratory. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIH Postdoctoral Fellowship1 F32CA05748-01
NIH Predoctoral FellowshipUNSPECIFIED
Subject Keywords:nonblue copper, copper proteins, electron paramagnetic resonance, square planar geometry, nitrogenous ligands
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Other Numbering System NameOther Numbering System ID
Arthur Amos Noyes Laboratory of Chemical Physics5801
Issue or Number:9
PubMed Central ID:PMC336053
Record Number:CaltechAUTHORS:DAWpnas78
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1118
Deposited By: Tony Diaz
Deposited On:20 Dec 2005
Last Modified:22 Nov 2019 09:58

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