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Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions

O’Toole, George A. and Crabbé, Aurélie and Kümmerli, Rolf and LiPuma, John J. and Bomberger, Jennifer M. and Davies, Jane C. and Limoli, Dominique and Phelan, Vanessa V. and Bliska, James B. and DePas, William H. and Dietrich, Lars E. and Hampton, Thomas H. and Hunter, Ryan and Khursigara, Cezar M. and Price-Whelan, Alexa and Ashare, Alix and Cramer, Robert A. and Goldberg, Joanna B. and Harrison, Freya and Hogan, Deborah A. and Henson, Michael A. and Madden, Dean R. and Mayers, Jared R. and Nadell, Carey and Newman, Dianne and Prince, Alice and Rivett, Damian W. and Schwartzman, Joseph D. and Schultz, Daniel and Sheppard, Donald C. and Smyth, Alan R. and Spero, Melanie A. and Stanton, Bruce A. and Turner, Paul E. and van der Gast, Chris and Whelan, Fiona J. and Whitaker, Rachel and Whiteson, Katrine (2021) Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions. mBio, 12 (5). Art. No. mBio.01763-21. ISSN 2150-7511. PMCID PMC8546538. doi:10.1128/mbio.01763-21.

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A recent workshop titled “Developing Models to Study Polymicrobial Infections,” sponsored by the Dartmouth Cystic Fibrosis Center (DartCF), explored the development of new models to study the polymicrobial infections associated with the airways of persons with cystic fibrosis (CF). The workshop gathered 35+ investigators over two virtual sessions. Here, we present the findings of this workshop, summarize some of the challenges involved with developing such models, and suggest three frameworks to tackle this complex problem. The frameworks proposed here, we believe, could be generally useful in developing new model systems for other infectious diseases. Developing and validating new approaches to study the complex polymicrobial communities in the CF airway could open windows to new therapeutics to treat these recalcitrant infections, as well as uncovering organizing principles applicable to chronic polymicrobial infections more generally.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
O’Toole, George A.0000-0002-2861-4392
Crabbé, Aurélie0000-0003-3084-4418
Kümmerli, Rolf0000-0003-4084-6679
LiPuma, John J.0000-0003-4033-7794
Bomberger, Jennifer M.0000-0003-4767-6238
Limoli, Dominique0000-0002-4130-337X
Phelan, Vanessa V.0000-0001-7156-9294
Bliska, James B.0000-0002-6047-8837
DePas, William H.0000-0001-8532-2567
Dietrich, Lars E.0000-0003-2049-1137
Hampton, Thomas H.0000-0003-0543-402X
Hunter, Ryan0000-0003-3841-1676
Khursigara, Cezar M.0000-0001-9263-7604
Price-Whelan, Alexa0000-0003-0872-7098
Cramer, Robert A.0000-0001-5503-5006
Goldberg, Joanna B.0000-0002-5285-5188
Hogan, Deborah A.0000-0002-6366-2971
Madden, Dean R.0000-0002-1810-6984
Mayers, Jared R.0000-0002-8607-1787
Nadell, Carey0000-0003-1751-4895
Newman, Dianne0000-0003-1647-1918
Prince, Alice0000-0002-7399-9295
Rivett, Damian W.0000-0002-1852-6137
Sheppard, Donald C.0000-0001-8877-880X
Smyth, Alan R.0000-0001-5494-5438
Spero, Melanie A.0000-0003-3291-2138
Stanton, Bruce A.0000-0002-1661-407X
van der Gast, Chris0000-0003-1101-4048
Whelan, Fiona J.0000-0001-9165-4859
Whitaker, Rachel0000-0003-2263-5798
Whiteson, Katrine0000-0002-5423-6014
Additional Information:© 2021 O’Toole et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Published 21 September 2021. We thank the Cystic Fibrosis Foundation’s willingness to support the original, in-person version of this workshop, which needed to be canceled and rescheduled as a virtual event due to the COVID-19 pandemic. We thank Madison Leonard and Cindy Stewart of DartCF for their administrative support, Fabrice Jean-Pierre for his assistance, and the workshop session moderators (A. Crabbé, J. Bomberger, V. Phelan, J. Davies, D. Limoli, R. Kümmerli) and scribes (T. Hampton, R. Hunter, L. Dietrich, A. Price-Whelan, C. Khursigara, J. Bliska, W. DePas) for their willingness to help both the planning of and logistical support during the workshop. DartCF is supported by the NIH (P30-DK117469) and the Cystic Fibrosis Foundation Research Development Program (STANTO19R0). Additional support was provided by NIH/R01AI155424, NIH/1R01HL152190, CFF/CRAMER19GO, CIHR/PJT-156111, NIH/R35GM128690, NIH/R01DK104847, R01/NIHAI127548, CFF/TURNER19PO, NIH/R01HL136919, CFF/ASHARE21G0, NIH/R35HL18500, NIH/P20GM130454, NIHR Systematic Reviews Program, CFF/WHITEL20A0, NIH/R01AI103369, University of Nottingham Anne McLaren Fellowship, Swiss National Science Foundation/31003A_182499, MRC/MR/R001898/1, NIH/R01HL151385, NIH/R01AI155424, CFF/BOMBER21R3, CFF/SPERO19F0, NIH/R01HL136647, Paul G. Allen Frontiers Investigator Award, NIH/R01HL136647, Cystic Fibrosis Trust Strategic Research Centre Award, CF Trust/VIA078, CF Trust/VIAO77, CIHR/FDN/159902, and CFF/BOMBER18G0.
Funding AgencyGrant Number
Cystic Fibrosis FoundationSTANTO19R0
Cystic Fibrosis FoundationCRAMER19GO
Canadian Institutes of Health Research (CIHR)PJT-156111
Cystic Fibrosis FoundationTURNER19PO
Cystic Fibrosis FoundationASHARE21G0
National Institute for Health ResearchUNSPECIFIED
Cystic Fibrosis FoundationWHITEL20A0
University of NottinghamUNSPECIFIED
Swiss National Science Foundation (SNSF)31003A_182499
Medical Research Council (UK)MR/R001898/1
Cystic Fibrosis FoundationBOMBER21R3
Cystic Fibrosis FoundationSPERO19F0
Paul G. Allen Family FoundationUNSPECIFIED
Cystic Fibrosis FoundationVIA078
Cystic Fibrosis FoundationVIAO77
Canadian Institutes of Health Research (CIHR)FDN/159902
Cystic Fibrosis FoundationBOMBER18G0
Subject Keywords:cystic fibrosis, models, polymicrobial, airway, chronic infection
Issue or Number:5
PubMed Central ID:PMC8546538
Record Number:CaltechAUTHORS:20211116-212551206
Persistent URL:
Official Citation:O’Toole GA, Crabbé A, Kümmerli R, LiPuma JJ, Bomberger JM, Davies JC, Limoli D, Phelan VV, Bliska JB, DePas WH, Dietrich LE, Hampton TH, Hunter R, Khursigara CM, Price- Whelan A, Ashare A, Cramer RA, Goldberg JB, Harrison F, Hogan DA, Henson MA, Madden DR, Mayers JR, Nadell C, Newman D, Prince A, Rivett DW, Schwartzman JD, Schultz D, Sheppard DC, Smyth AR, Spero MA, Stanton BA, Turner PE, van der Gast C, Whelan FJ, Whitaker R, Whiteson K. 2021. Model systems to study the chronic, polymicrobial infections in cystic fibrosis: current approaches and exploring future directions. mBio 12:e01763-21.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:111911
Deposited By: Tony Diaz
Deposited On:16 Nov 2021 22:03
Last Modified:16 Nov 2021 22:03

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