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Conjugated Bio-Polymer Anchored Surfaces to Mitigate Stain and Bacterial Colonization for Oral Hygiene Application

Pathan, Shabnam and Samantaray, Paresh Kumar and Nilawar, Sagar and Roychowdhury, Sumana and Das, Somnath and Samadder, Satyajit and Pramanik, Amitava and Chatterjee, Kaushik and Bose, Suryasarathi (2021) Conjugated Bio-Polymer Anchored Surfaces to Mitigate Stain and Bacterial Colonization for Oral Hygiene Application. ACS Applied Polymer Materials, 3 (10). pp. 4812-4824. ISSN 2637-6105. doi:10.1021/acsapm.0c01323. https://resolver.caltech.edu/CaltechAUTHORS:20211122-173948795

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Abstract

Due to health and environmental hazards, there is an increased desire to rely on sustainable resources that are biocompatible, biodegradable, and safe for human administration. Herein, the present study explores bioconjugate efficacy as a potential anti-stain and green antibacterial additive in toothpaste formulation using GRAS (generally regarded as safe) and Food and Drug Administration (FDA)-approved ingredients. In this study, a series of conjugated polymers were synthesized using sodium alginate and alendronic acid and are labeled as SA-ALN amide and SA-ALN ester. Spectroscopic techniques thoroughly characterized the synthesized conjugated polymers. These synthesized polymers were adsorbed onto hydroxyapatite (HAP, a tooth model) to evaluate its practical application as anti-stain and reduce bacterial attachment. The adsorption of the conjugate polymers on the HAP surface was confirmed by Raman spectra and Fourier transform infrared spectroscopy (FTIR). The anti-stain test revealed significant whitening benefits for coated hydroxyapatite (HAP) tiles over uncoated tiles. The antibacterial activity of conjugated polymers toward E. coli (Gram-negative) and S. mutans (Gram-positive, bacteria responsible for tooth decay) strain showed 2.5 log reduction and 1.5 log reduction, respectively. Among the conjugated polymers, the SA-ALN-ester showed slightly higher antibacterial activity against E. coli as well as S. mutans strain relative to the SA-ALN amide product due to the presence of a free amine group in the former. Further, we carried out the in vitro cytotoxicity test of the conjugated polymers using gingival fibroblasts and pre-osteoblasts and the study demonstrated that the synthesized conjugates are cytocompatible in nature. Thus, this study revealed that the proposed work might provide insights into sustainable oral hygiene.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acsapm.0c01323DOIArticle
ORCID:
AuthorORCID
Pathan, Shabnam0000-0002-5869-0150
Samantaray, Paresh Kumar0000-0003-2533-929X
Pramanik, Amitava0000-0002-4606-0979
Chatterjee, Kaushik0000-0002-7204-2926
Bose, Suryasarathi0000-0001-8043-9192
Additional Information:© 2021 American Chemical Society. Received: November 28, 2020; Published: September 14, 2021. S.P. is grateful to Hindustan Unilever Research Center, Bengaluru, for funding (SID/PC-99254) this study. Authors would like to acknowledge Prof P. Kondaiah for the cells. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
UnileverSID/PC-99254
Subject Keywords:sodium alginate, alendronic acid, hydroxyapatite, live/dead assay, reactive oxygen species
Issue or Number:10
DOI:10.1021/acsapm.0c01323
Record Number:CaltechAUTHORS:20211122-173948795
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20211122-173948795
Official Citation:Conjugated Bio-Polymer Anchored Surfaces to Mitigate Stain and Bacterial Colonization for Oral Hygiene Application. Shabnam Pathan, Paresh Kumar Samantaray, Sagar Nilawar, Sumana Roychowdhury, Somnath Das, Satyajit Samadder, Amitava Pramanik, Kaushik Chatterjee, and Suryasarathi Bose. ACS Applied Polymer Materials 2021 3 (10), 4812-4824; DOI: 10.1021/acsapm.0c01323
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:111971
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Nov 2021 17:56
Last Modified:22 Nov 2021 17:56

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