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Phagocytosis of carbohydrate-modified phospholipid vesicles by macrophage

Wu, Po-shun and Tin, George W. and Baldeschwieler, John D. (1981) Phagocytosis of carbohydrate-modified phospholipid vesicles by macrophage. Proceedings of the National Academy of Sciences of the United States of America, 78 (4). pp. 2033-2037. ISSN 0027-8424.

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Modification of the surface of distearoyl phosphatidylcholine vesicles with synthetic glycolipids dramatically affects the rate of uptake of these vesicles by mouse peritoneal macrophage. The high rate of uptake of 6-aminomannose-modified vesicles is effectively inhibited by cytochalasin B and chloroquine but not by colchicine, indicating that the mechanism of vesicle uptake is phagocytosis. Other modified vesicles appear to have some effect on the rate of uptake of 6-aminomannose-modified vesicles suggesting that the various vesicle types compete for the same initial binding sites. Analysis of 6-aminomannose-modified vesicles by γ-ray perturbed angular correlation spectroscopy shows that the rotational correlation time of the encapsulated (111)In(3+) does not change when the vesicles associate with macrophage. This result is consistent with transmission electron microscopy, which indicates that the aminomannose-modified vesicles remain intact after phagocytosis as aggregates of fused and intact vesicles surrounded by a single bilayer membrane structure.

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Additional Information:Copyright © 1981 by the National Academy of Sciences. Contributed by John D. Baldeschwieler, November 20, 1980. We thank Drs. T. Y. Shen and M. M. Ponpipom (Merck Sharp & Dohme) and Drs. William S. Sly and Paul H. Schlesinger (Washington University School of Medicine, St. Louis, MO) for valuable discussions during the course of these studies. This work was supported by National Institutes of Health Grant GM 21111-07, National Science Foundation Grant CHE79-18401, and a grant from Merck & Co., Inc. This is contribution no. 6342 from the Arthur Amos Noyes Laboratory of Chemical Physics. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:liposomes, perturbed angular correlation spectroscopy, cell-surface receptor, drug-delivery systems
Issue or Number:4
Record Number:CaltechAUTHORS:WUPpnas81a
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1131
Deposited By: Tony Diaz
Deposited On:22 Dec 2005
Last Modified:02 Oct 2019 22:40

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