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In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography

Zhao, Wei and Jensen, Grant J. (2022) In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220222-705804000

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Abstract

AbstractFaithful segregation of chromosomes during mitosis relies on a carefully coordinated and intricate interplay between the centromere, kinetochore, and spindle microtubules. Despite its importance, the architecture of this interface remains elusive. Here we used in situ cryo-electron tomography to visualize the native architecture of the kinetochore-microtubule interface in human U2OS cells at different stages of mitosis. We find that the centromere forms a pocket-like structure around kinetochore microtubules. Two morphologically distinct fibrillar densities form end-on and side-on connections to the plus-ends of microtubules within this centromeric pocket. Our data suggest a dynamic kinetochore-microtubule interface with multiple interactions between outer kinetochore components and spindle microtubules.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2022.02.17.480955DOIArticle
https://etdb.caltech.edu/Related ItemCaltech Electron Tomography Database
ORCID:
AuthorORCID
Zhao, Wei0000-0002-4441-9722
Jensen, Grant J.0000-0003-1556-4864
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license. : We thank Dr. Songye Chen and Dr. Andrey Malyutin for technical assistance with cryo-electron microscopy. We thank Dr. Catherine M. Oikonomou for her very helpful comments on the manuscript. Cryo-electron microscopy was performed in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. This work was supported by NIH grant P50 AI150464 to G.J.J. Author contributions: W.Z. and G.J.J. conceived of the project. W.Z. and G.J.J. designed the experiments. W.Z. performed and interpreted the experiments. W.Z. and G.J.J. wrote the paper. Data and materials availability: All data is available upon request. Tomograms will be made publicly available through the Caltech Electron Tomography Database at https://etdb.caltech.edu. The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
NIHP50 AI150464
DOI:10.1101/2022.02.17.480955
Record Number:CaltechAUTHORS:20220222-705804000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220222-705804000
Official Citation:In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography Wei Zhao, Grant J. Jensen bioRxiv 2022.02.17.480955; doi: https://doi.org/10.1101/2022.02.17.480955
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113519
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:24 Feb 2022 17:43
Last Modified:24 Feb 2022 17:43

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