Zhao, Wei and Jensen, Grant J. (2022) In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220222-705804000
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Abstract
AbstractFaithful segregation of chromosomes during mitosis relies on a carefully coordinated and intricate interplay between the centromere, kinetochore, and spindle microtubules. Despite its importance, the architecture of this interface remains elusive. Here we used in situ cryo-electron tomography to visualize the native architecture of the kinetochore-microtubule interface in human U2OS cells at different stages of mitosis. We find that the centromere forms a pocket-like structure around kinetochore microtubules. Two morphologically distinct fibrillar densities form end-on and side-on connections to the plus-ends of microtubules within this centromeric pocket. Our data suggest a dynamic kinetochore-microtubule interface with multiple interactions between outer kinetochore components and spindle microtubules.
| Item Type: | Report or Paper (Discussion Paper) | |||||||||
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| Additional Information: | The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license. : We thank Dr. Songye Chen and Dr. Andrey Malyutin for technical assistance with cryo-electron microscopy. We thank Dr. Catherine M. Oikonomou for her very helpful comments on the manuscript. Cryo-electron microscopy was performed in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. This work was supported by NIH grant P50 AI150464 to G.J.J. Author contributions: W.Z. and G.J.J. conceived of the project. W.Z. and G.J.J. designed the experiments. W.Z. performed and interpreted the experiments. W.Z. and G.J.J. wrote the paper. Data and materials availability: All data is available upon request. Tomograms will be made publicly available through the Caltech Electron Tomography Database at https://etdb.caltech.edu. The authors have declared no competing interest. | |||||||||
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| DOI: | 10.1101/2022.02.17.480955 | |||||||||
| Record Number: | CaltechAUTHORS:20220222-705804000 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220222-705804000 | |||||||||
| Official Citation: | In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography Wei Zhao, Grant J. Jensen bioRxiv 2022.02.17.480955; doi: https://doi.org/10.1101/2022.02.17.480955 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 113519 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 24 Feb 2022 17:43 | |||||||||
| Last Modified: | 24 Feb 2022 17:43 |
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