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In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography

Zhao, Wei and Jensen, Grant J. (2022) In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography. . (Unpublished)

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AbstractFaithful segregation of chromosomes during mitosis relies on a carefully coordinated and intricate interplay between the centromere, kinetochore, and spindle microtubules. Despite its importance, the architecture of this interface remains elusive. Here we used in situ cryo-electron tomography to visualize the native architecture of the kinetochore-microtubule interface in human U2OS cells at different stages of mitosis. We find that the centromere forms a pocket-like structure around kinetochore microtubules. Two morphologically distinct fibrillar densities form end-on and side-on connections to the plus-ends of microtubules within this centromeric pocket. Our data suggest a dynamic kinetochore-microtubule interface with multiple interactions between outer kinetochore components and spindle microtubules.

Item Type:Report or Paper (Discussion Paper)
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URLURL TypeDescription ItemCaltech Electron Tomography Database
Zhao, Wei0000-0002-4441-9722
Jensen, Grant J.0000-0003-1556-4864
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license. : We thank Dr. Songye Chen and Dr. Andrey Malyutin for technical assistance with cryo-electron microscopy. We thank Dr. Catherine M. Oikonomou for her very helpful comments on the manuscript. Cryo-electron microscopy was performed in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. This work was supported by NIH grant P50 AI150464 to G.J.J. Author contributions: W.Z. and G.J.J. conceived of the project. W.Z. and G.J.J. designed the experiments. W.Z. performed and interpreted the experiments. W.Z. and G.J.J. wrote the paper. Data and materials availability: All data is available upon request. Tomograms will be made publicly available through the Caltech Electron Tomography Database at The authors have declared no competing interest.
Funding AgencyGrant Number
NIHP50 AI150464
Record Number:CaltechAUTHORS:20220222-705804000
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Official Citation:In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography Wei Zhao, Grant J. Jensen bioRxiv 2022.02.17.480955; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113519
Deposited By: George Porter
Deposited On:24 Feb 2022 17:43
Last Modified:24 Feb 2022 17:43

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