A Caltech Library Service

DiI staining of sensory neurons in the entomopathogenic nematode Steinernema hermaphroditum

Garg, Pranjal and Tan, Chieh-Hsiang and Sternberg, Paul W. (2022) DiI staining of sensory neurons in the entomopathogenic nematode Steinernema hermaphroditum. microPublication Biology . ISSN 2578-9430. PMCID PMC8874337.

[img] PDF - Published Version
Creative Commons Attribution.


Use this Persistent URL to link to this item:


Steinernema hermaphroditum entomopathogenic nematodes (EPN) and their Xenorhabdus griffiniae symbiotic bacteria have recently been shown to be a genetically tractable system for the study of both parasitic and mutualistic symbiosis. In their infective juvenile (IJ) stage, EPNs search for insect hosts to invade and quickly kill them with the help of the symbiotic bacteria they contain. The mechanisms behind these behaviors have not been well characterized, including how the nematodes sense their insect hosts. In the well-studied free‑living soil nematode Caenorhabditis elegans, ciliated amphid neurons enable the worms to sense their environment, including chemosensation. Some of these neurons have also been shown to control the decision to develop as a stress-resistant dauer larva, analogous to the infective juveniles of EPNs, or to exit from dauer and resume larval development. In C. elegans and other nematodes, dye-filling with DiI is an easy and efficient method to label these neurons. We developed a protocol for DiI staining of S. hermaphroditum sensory neurons. Using this method, we could identify neurons positionally analogous to the C. elegans amphid neurons ASI, ADL, ASK, ASJ, as well as inner labial neurons IL1 and IL2. Similar to findings in other EPNs, we also found that the IJs of S. hermaphroditum are dye-filling resistant.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Tan, Chieh-Hsiang0000-0002-5432-0160
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2022 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. History: Received January 12, 2022 Revision received January 26, 2022 Accepted February 10, 2022 Published February 24, 2022. Funding: NSF EDGE grant 2128267 to PWS. Author Contributions: Pranjal Garg: Formal analysis, Investigation, Methodology, Visualization, Writing - original draft. Chieh-Hsiang Tan: Conceptualization, Methodology, Supervision, Writing - original draft. Paul W. Sternberg: Conceptualization, Funding acquisition, Supervision, Writing - review and editing. Reviewed By: Ray Hong.
Funding AgencyGrant Number
PubMed Central ID:PMC8874337
Record Number:CaltechAUTHORS:20220228-618839000
Persistent URL:
Official Citation:Garg, P; Tan, CH; Sternberg, PW (2022). DiI staining of sensory neurons in the entomopathogenic nematode Steinernema hermaphroditum. microPublication Biology. 10.17912/micropub.biology.000516.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113620
Deposited By: George Porter
Deposited On:28 Feb 2022 21:40
Last Modified:28 Feb 2022 21:40

Repository Staff Only: item control page