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Nanobiolistics: An Emerging Genetic Transformation Approach

Cunningham, Francis J. and Demirer, Gozde S. and Goh, Natalie S. and Zhang, Huan and Landry, Markita P. (2020) Nanobiolistics: An Emerging Genetic Transformation Approach. In: Biolistic DNA Delivery in Plants. Methods in Molecular Biology. No.2124. Humana Press , New York, NY, pp. 141-159. ISBN 9781071603550.

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Biolistic delivery of biomolecular cargoes to plants with micron-scale projectiles is a well-established technique in plant biotechnology. However, the relatively large micron-scale biolistic projectiles can result in tissue damage, low regeneration efficiency, and create difficulties for the biolistic transformation of isomorphic small cells or subcellular target organelles (i.e., mitochondria and plastids). As an alternative to micron-sized carriers, nanomaterials provide a promising approach for biomolecule delivery to plants. While most studies exploring nanoscale biolistic carriers have been carried out in animal cells and tissues, which lack a cell wall, we can nonetheless extrapolate their utility for nanobiolistic delivery of biomolecules in plant targets. Specifically, nanobiolistics has shown promising results for use in animal systems, in which nanoscale projectiles yield lower levels of cell and tissue damage while maintaining similar transformation efficiencies as their micron-scale counterparts. In this chapter, we specifically discuss biolistic delivery of nanoparticles for plant genetic transformation purposes and identify the figures of merit requiring optimization for broad-scale implementation of nanobiolistics in plant genetic transformations.

Item Type:Book Section
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Demirer, Gozde S.0000-0002-3007-1489
Landry, Markita P.0000-0002-5832-8522
Additional Information:© Springer Science+Business Media, LLC, part of Springer Nature 2020. Francis J. Cunningham, Gozde S. Demirer, Natalie S. Goh, and Huan Zhang contributed equally to this work. The authors acknowledge support from a Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI), a Beckman Foundation Young Investigator Award, a USDA AFRI award, a grant from the Gordon and Betty Moore Foundation, a USDA NIFA award, an NIH MIRA award, support from the Chan-Zuckerberg foundation, and an FFAR New Innovator Award (to M.P.L). F.J.C is supported by an NSF Graduate Research Fellowship, N.S.G is supported by a FFAR Fellowship, and G.S.D. is supported by a Schlumberger Foundation Faculty for the Future Fellowship.
Funding AgencyGrant Number
Burroughs Wellcome FundUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Department of AgricultureUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Chan-Zuckerberg FoundationUNSPECIFIED
Foundation for Food ResearchUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
Schlumberger FoundationUNSPECIFIED
Subject Keywords:Biolistics; Nanobiolistics; Plant transformation; Agriculture; Bionanotechnology; Nanoparticles; Gold nanoparticles; Mesoporous silica nanoparticles (MSNs); Carbon nanotubes (CNTs)
Series Name:Methods in Molecular Biology
Issue or Number:2124
Record Number:CaltechAUTHORS:20220302-323890000
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113702
Deposited By: George Porter
Deposited On:03 Mar 2022 16:49
Last Modified:03 Mar 2022 16:49

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