Li, Lei and Verkhusha, Vladislav V. and Shcherbakova, Daria M. and Wang, Lihong V. (2022) Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor. In: Photons Plus Ultrasound: Imaging and Sensing 2022. Proceedings of SPIE. No.11960. Society of Photo-optical Instrumentation Engineers , Bellingham, WA, Art. No. 119600J. https://resolver.caltech.edu/CaltechAUTHORS:20220307-189707000
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Abstract
Photoacoustic tomography with genetically encoded near-infrared probes enables visualization of specific cell populations in vivo at high resolution in deep tissues. Here, we report a near-infrared Forster resonance energy transfer biosensor based on a pair of the near-infrared fluorescent proteins, miRFP670-iRFP720, which enables dynamic photoacoustic imaging of active biological processes. Specifically, we detected apoptosis in single cells at a resolution of ~3 µm in a mouse ear and mouse brain tumors (>3 mm in depth) at a spatial resolution of ~150 µm. These results open the way for high-resolution photoacoustic imaging of dynamic biological processes in deep tissues.
| Item Type: | Book Section | ||||||||||
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| Additional Information: | © 2022 Society of Photo-Optical Instrumentation Engineers (SPIE). | ||||||||||
| Subject Keywords: | PAT, photoacoustic microscopy, FRET, biosensor | ||||||||||
| Series Name: | Proceedings of SPIE | ||||||||||
| Issue or Number: | 11960 | ||||||||||
| DOI: | 10.1117/12.2610587 | ||||||||||
| Record Number: | CaltechAUTHORS:20220307-189707000 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220307-189707000 | ||||||||||
| Official Citation: | Lei Li, Lihong Wang, Vladislav V. Verkhusha, and Daria Shcherbakova "Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor", Proc. SPIE 11960, Photons Plus Ultrasound: Imaging and Sensing 2022, 119600J (3 March 2022); https://doi.org/10.1117/12.2610587 | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 113785 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | George Porter | ||||||||||
| Deposited On: | 08 Mar 2022 21:26 | ||||||||||
| Last Modified: | 08 Mar 2022 21:26 |
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