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Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor

Li, Lei and Verkhusha, Vladislav V. and Shcherbakova, Daria M. and Wang, Lihong V. (2022) Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor. In: Photons Plus Ultrasound: Imaging and Sensing 2022. Proceedings of SPIE. No.11960. Society of Photo-optical Instrumentation Engineers , Bellingham, WA, Art. No. 119600J. https://resolver.caltech.edu/CaltechAUTHORS:20220307-189707000

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Abstract

Photoacoustic tomography with genetically encoded near-infrared probes enables visualization of specific cell populations in vivo at high resolution in deep tissues. Here, we report a near-infrared Forster resonance energy transfer biosensor based on a pair of the near-infrared fluorescent proteins, miRFP670-iRFP720, which enables dynamic photoacoustic imaging of active biological processes. Specifically, we detected apoptosis in single cells at a resolution of ~3 µm in a mouse ear and mouse brain tumors (>3 mm in depth) at a spatial resolution of ~150 µm. These results open the way for high-resolution photoacoustic imaging of dynamic biological processes in deep tissues.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2610587DOIArticle
ORCID:
AuthorORCID
Li, Lei0000-0001-6164-2646
Verkhusha, Vladislav V.0000-0002-2083-8121
Shcherbakova, Daria M.0000-0003-3384-6363
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2022 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:PAT, photoacoustic microscopy, FRET, biosensor
Series Name:Proceedings of SPIE
Issue or Number:11960
DOI:10.1117/12.2610587
Record Number:CaltechAUTHORS:20220307-189707000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220307-189707000
Official Citation:Lei Li, Lihong Wang, Vladislav V. Verkhusha, and Daria Shcherbakova "Multiscale photoacoustic tomography of a genetically encoded near-infrared FRET biosensor", Proc. SPIE 11960, Photons Plus Ultrasound: Imaging and Sensing 2022, 119600J (3 March 2022); https://doi.org/10.1117/12.2610587
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113785
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Mar 2022 21:26
Last Modified:08 Mar 2022 21:26

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