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Cell Chromatography: Biocompatible chromatographic separation and interrogation of microbial cells

Hazu, M. and Ahmed, A. and Curry, E. and Hornby, D. P. and Gjerde, D. T. (2022) Cell Chromatography: Biocompatible chromatographic separation and interrogation of microbial cells. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220308-543763000

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Abstract

The isolation of pure, single colonies lies at the heart of experimental microbiology. However, a microbial colony typically contains around one million cells at all stages of the life cycle. Here we describe a novel, cell chromatography method that facilitates the capture, purification and interrogation of microbial cells from both single and mixed cultures. The method described relies on, but is not limited to, differences in surface charge to separate bacterial strains. The method is fully biocompatible, leading to no significant loss of cell viability, . The chromatographic capture of cells, combined with selective elution methods facilitates a greater level of experimental control over the sample inputs required for downstream high throughput and high sensitivity, analytical methods. The application of the method for interrogating the antibiotic resistance of bacterial strains and for the separation of bacteria from environmental samples is illustrated.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2022.03.07.482710DOIDiscussion Paper
https://resolver.caltech.edu/CaltechAUTHORS:20221003-756400000.20Related ItemJournal article
ORCID:
AuthorORCID
Hazu, M.0000-0002-6527-9597
Hornby, D. P.0000-0002-8188-7023
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. The authors have declared no competing interest.
DOI:10.1101/2022.03.07.482710
Record Number:CaltechAUTHORS:20220308-543763000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220308-543763000
Official Citation:Cell Chromatography: Biocompatible chromatographic separation and interrogation of microbial cells Masami Hazu, Ashraf Ahmed, Edward AJ Curry, David P Hornby, Douglas T Gjerde bioRxiv 2022.03.07.482710; doi: https://doi.org/10.1101/2022.03.07.482710
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113794
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Mar 2022 15:09
Last Modified:04 Oct 2022 23:50

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