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Stress-tolerant, recyclable, and autonomously renewable biocatalyst platform enabled by engineered bacterial spores

Hui, Yue and Cui, Ziyu and Sim, Seunghyun (2022) Stress-tolerant, recyclable, and autonomously renewable biocatalyst platform enabled by engineered bacterial spores. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220322-742124000

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Abstract

Here, we describe a stress-tolerant, recyclable, and autonomously renewable biocatalyst platform based on T7 RNA polymerase-enabled high-density protein display on bacterial spores (TIED). TIED uses high-level T7 RNA polymerase-driven expression of recombinant proteins specifically in sporulating cells to allow spontaneous assembly of recombinant fusion proteins on B. subtilis spore surface. TIED enables a high loading density in the range of 10⁶–10⁷ recombinant enzymes per spore, robust catalytic activities of displayed enzymes comparable to the respective free enzymes, and enhanced kinetic stability of displayed enzymes in methanol and elevated temperatures. Further, we demonstrate TIED-enzymes to be not only recyclable, but fully renewable after loss of activity through induction of germination and sporulation, enabling perpetual reuse of these immobilized biocatalysts.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2022.03.16.484680DOIDiscussion Paper
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This work is supported by start-up funds (S. S.) from the University of California, Irvine. The authors thank Professor David Tirrell for his helpful comments. AUTHOR CONTRIBUTIONS. Y. H. and S. S. designed experiments, analyzed the data, and wrote the manuscript. Y.H. and Z. C. performed the experiments and analyzed the data. The authors declare no competing financial interests.
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University of California, IrvineUNSPECIFIED
DOI:10.1101/2022.03.16.484680
Record Number:CaltechAUTHORS:20220322-742124000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220322-742124000
Official Citation:Stress-tolerant, recyclable, and autonomously renewable biocatalyst platform enabled by engineered bacterial spores Yue Hui, Ziyu Cui, Seunghyun Sim bioRxiv 2022.03.16.484680; doi: https://doi.org/10.1101/2022.03.16.484680
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:113998
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Mar 2022 19:21
Last Modified:23 Mar 2022 19:21

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