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Enzyme Nanorings

Chou, Tsui-Fen and So, Christopher and White, Brian R. and Carlson, Jonathan C. T. and Sarikaya, Mehmet and Wagner, Carston R. (2008) Enzyme Nanorings. ACS Nano, 2 (12). pp. 2519-2525. ISSN 1936-0851. PMCID PMC2682639. doi:10.1021/nn800577h.

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We have demonstrated that nanostructures, and in particular nanorings incorporating a homodimeric enzyme, can be prepared by chemically induced self-assembly of dihydrofolate reductase (DHFR)-histidine triad nucleotide binding 1 (Hint1) fusion proteins. The dimensions of the nanorings were found by static light scattering and atomic force microscopy studies to be dependent on the length and composition of the peptide linking the fusion proteins, ranging in size from 10 to 70 nm in diameter and 64 to 740 kDa. The catalytic efficiency of the nanorings was found to be dependent on ring size, thus suggesting that the arrangement of supermolecular assemblies of enzymes may be used to control their catalytic parameters.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Chou, Tsui-Fen0000-0003-2410-2186
So, Christopher0000-0002-1393-0333
Carlson, Jonathan C. T.0000-0003-4139-9057
Sarikaya, Mehmet0000-0003-3856-6360
Wagner, Carston R.0000-0001-7927-719X
Additional Information:© 2008 American Chemical Society. Received 14 September 2008. Accepted 22 November 2008. Published online 9 December 2008. Published in issue 23 December 2008. We thank Dr. Terry W. Steele for his assistance with the SLS experiments, and Dr. R. A. Siegel for the use of the SLS instrument. We wish to thank the University of Minnesota Nanobiotechnology Initiative (C.R.W.), NIH-NCI (CA120116, C.R.W.), the Leukemia Research Foundation, and NSF-MRSEC (C.S. and M.S.) for partial support of this study (DMR-0520567).
Funding AgencyGrant Number
University of MinnesotaUNSPECIFIED
Leukemia Research FoundationUNSPECIFIED
Subject Keywords:Peptides and proteins, Monomers, Cyclization, Oligomers, Macrocycles
Issue or Number:12
PubMed Central ID:PMC2682639
Record Number:CaltechAUTHORS:20220323-565456000
Persistent URL:
Official Citation:Enzyme Nanorings Tsui-Fen Chou, Christopher So, Brian R. White, Jonathan C. T. Carlson, Mehmet Sarikaya, and Carston R. Wagner ACS Nano 2008 2 (12), 2519-2525 DOI: 10.1021/nn800577h
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:114034
Deposited By: George Porter
Deposited On:25 Mar 2022 17:04
Last Modified:25 Mar 2022 17:04

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