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Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter

Han, Yifu and Ding, Keke (2022) Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter. In: Synaptic Vesicles. Methods in Molecular Biology. No.2417. Humana Press , New York, NY, pp. 193-203. ISBN 978-1-0716-1915-5.

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Despite the important roles of neuropeptides in a variety of physiological processes, there still lacks a method to probe neuropeptide release events in vivo with satisfying temporal and spatial resolution. Neuropeptide Release Reporter (NPRR) was recently introduced as a novel genetically encoded indicator of neuropeptide release with a high temporal resolution and peptide specificity based on GCaMP molecule. Here we describe a method for using NPRR to image selective neuropeptide release at Drosophila neuromuscular junction in semi-dissected larvae. This method provides a quantitative analysis of activity-dependent neuropeptide release as real-time changes in fluorescence intensity of GCaMP reporter with sub-second temporal resolution and single bouton specificity.

Item Type:Book Section
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URLURL TypeDescription
Han, Yifu0000-0002-1201-654X
Ding, Keke0000-0002-5261-4843
Additional Information:© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. Work in the Dickman lab is funded by a grant from the National Institutes of Health (NS091546). We thank Taylor Seid (California Institute of Technology), David Anderson (California Institute of Technology), and Dion Dickman (University of Southern California) for insightful discussions and comments. The authors declare no conflicts or other competing interests.
Funding AgencyGrant Number
Subject Keywords:Drosophila; Neuromuscular junction; Dense core vesicle; Neuropeptide release; Calcium imaging
Series Name:Methods in Molecular Biology
Issue or Number:2417
Record Number:CaltechAUTHORS:20220413-42551000
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:114286
Deposited By: Tony Diaz
Deposited On:13 Apr 2022 21:35
Last Modified:13 Apr 2022 21:35

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