Han, Yifu and Ding, Keke (2022) Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter. In: Synaptic Vesicles. Methods in Molecular Biology. No.2417. Humana Press , New York, NY, pp. 193-203. ISBN 978-1-0716-1915-5. https://resolver.caltech.edu/CaltechAUTHORS:20220413-42551000
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20220413-42551000
Abstract
Despite the important roles of neuropeptides in a variety of physiological processes, there still lacks a method to probe neuropeptide release events in vivo with satisfying temporal and spatial resolution. Neuropeptide Release Reporter (NPRR) was recently introduced as a novel genetically encoded indicator of neuropeptide release with a high temporal resolution and peptide specificity based on GCaMP molecule. Here we describe a method for using NPRR to image selective neuropeptide release at Drosophila neuromuscular junction in semi-dissected larvae. This method provides a quantitative analysis of activity-dependent neuropeptide release as real-time changes in fluorescence intensity of GCaMP reporter with sub-second temporal resolution and single bouton specificity.
| Item Type: | Book Section | ||||||
|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||
| ORCID: |
| ||||||
| Additional Information: | © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. Work in the Dickman lab is funded by a grant from the National Institutes of Health (NS091546). We thank Taylor Seid (California Institute of Technology), David Anderson (California Institute of Technology), and Dion Dickman (University of Southern California) for insightful discussions and comments. The authors declare no conflicts or other competing interests. | ||||||
| Funders: |
| ||||||
| Subject Keywords: | Drosophila; Neuromuscular junction; Dense core vesicle; Neuropeptide release; Calcium imaging | ||||||
| Series Name: | Methods in Molecular Biology | ||||||
| Issue or Number: | 2417 | ||||||
| DOI: | 10.1007/978-1-0716-1916-2_15 | ||||||
| Record Number: | CaltechAUTHORS:20220413-42551000 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220413-42551000 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 114286 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Tony Diaz | ||||||
| Deposited On: | 13 Apr 2022 21:35 | ||||||
| Last Modified: | 13 Apr 2022 21:35 |
Repository Staff Only: item control page
