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Electrochemical Modulation of Carbon Monoxide‐Mediated Cell Signaling

Park, Jimin and Zeng, Joy S. and Sahasrabudhe, Atharva and Jin, Kyoungsuk and Fink, Yoel and Manthiram, Karthish and Anikeeva, Polina (2021) Electrochemical Modulation of Carbon Monoxide‐Mediated Cell Signaling. Angewandte Chemie International Edition, 60 (37). pp. 20325-20330. ISSN 1433-7851. PMCID PMC8405587. doi:10.1002/anie.202103228. https://resolver.caltech.edu/CaltechAUTHORS:20220505-565109000

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Abstract

Despite the critical role played by carbon monoxide (CO) in physiological and pathological signaling events, current approaches to deliver this messenger molecule are often accompanied by off-target effects and offer limited control over release kinetics. To address these challenges, we develop an electrochemical approach that affords on-demand release of CO through reduction of carbon dioxide (CO₂) dissolved in the extracellular space. Electrocatalytic generation of CO by cobalt phthalocyanine molecular catalysts modulates signaling pathways mediated by a CO receptor soluble guanylyl cyclase. Furthermore, by tuning the applied voltage during electrocatalysis, we explore the effect of the CO release kinetics on CO-dependent neuronal signaling. Finally, we integrate components of our electrochemical platform into microscale fibers to produce CO in a spatially-restricted manner and to activate signaling cascades in the targeted cells. By offering on-demand local synthesis of CO, our approach may facilitate the studies of physiological processes affected by this gaseous molecular messenger.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.202103228DOIArticle
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fanie.202103228&file=anie202103228-sup-0001-misc_information.pdfPublisherSupporting Information
ORCID:
AuthorORCID
Park, Jimin0000-0002-7935-4340
Zeng, Joy S.0000-0002-3443-3504
Sahasrabudhe, Atharva0000-0002-2615-2470
Jin, Kyoungsuk0000-0003-3009-6691
Fink, Yoel0000-0001-9752-2283
Manthiram, Karthish0000-0001-9260-3391
Anikeeva, Polina0000-0001-6495-5197
Additional Information:© 2021 Wiley-VCH. Issue Online: 30 August 2021. Version of Record online: 11 August 2021. Accepted manuscript online: 15 July 2021. Manuscript received: 04 March 2021. We would like to thank T. Kitaguchi, and F. Zhang for the generous gifts of the plasmids and cell lines. The authors are also grateful to T. Khudiyev, S. Rao, and D. Rosenfeld for their technical advice on the experiments. This work was funded in part by the National Institutes of Health (NIH) BRAIN Initiative (5-R01-MH111872, 1-R01-NS115576), the National Institute of Neurological Disorders and Stroke (1-R01-NS115025), the National Science Foundation (NSF) Center for Neurotechnology (EEC-1028725), the Hock E. Tan and K. Lisa Yang Center for Autism Research, and the McGovern Institute for Brain Research. Work by K.M. and J.S.Z. was supported by the NSF under grant no. 1955628. This work made use of the MIT MRSEC Shared Experimental Facilities under award number DMR-14-19807 from the NSF. J.P. is a recipient of scholarship from the Kwanjeong Educational Foundation. A.S. is a recipient of the Lore Harp McGovern graduate student fellowship. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
NIH5-R01-MH111872
NIH1-R01-NS115576
NIH1-R01-NS115025
NSFEEC-1028725
Hock E. Tan and K. Lisa Yang Center for Autism ResearchUNSPECIFIED
McGovern Institute for Brain ResearchUNSPECIFIED
NSFCHE-1955628
NSFDMR-1419807
Kwanjeong Educational FoundationUNSPECIFIED
Subject Keywords:carbon monoxide; cell signaling; electrochemistry; fiber drawing; receptors
Issue or Number:37
PubMed Central ID:PMC8405587
DOI:10.1002/anie.202103228
Record Number:CaltechAUTHORS:20220505-565109000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220505-565109000
Official Citation:J. Park, J. S. Zeng, A. Sahasrabudhe, K. Jin, Y. Fink, K. Manthiram, P. Anikeeva, Angew. Chem. Int. Ed. 2021, 60, 20325.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:114609
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 May 2022 16:51
Last Modified:06 May 2022 16:51

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