DeGiorgis, Joseph A. and Jang, Marcus and Bearer, Elaine L. (2022) The Giant Axon of the Squid: A Simple System for Axonal Transport Studies. In: Axonal Transport: Methods and Protocols. Methods in Molecular Biology. No.2431. Humana Press , New York, NY, pp. 3-22. ISBN 978-1-0716-1989-6. https://resolver.caltech.edu/CaltechAUTHORS:20220511-183651271
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20220511-183651271
Abstract
The squid giant axon has a long history of being a superb experimental system in which to investigate a wide range of questions concerning intracellular transport. In this protocol we describe the method used for dissecting the axon to preserve its viability in vitro, and the technique for injecting exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-out squid genes, the giant axon will resume its place in the scientific pantheon of powerful experimental systems in which to address biological questions pertaining to all eukaryotes.
| Item Type: | Book Section | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||||||||
| ORCID: |
| ||||||||||||||
| Additional Information: | © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. First Online: 13 April 2022. We are grateful to Prasanna Satpute-Krishnan, Derek Nobrega, and Michael P. Conley for development of this protocol and some of the drawings, to Thomas S. Reese for inspiration and guidance, to Jim Galbraith for construction of the squid table, to Mark Terasaki and Laurinda Jaffe for injection advice, and to the Marine Biological Laboratory Embryology Course for loan of the micromanipulator and other injection equipment. We also thank Paulette Ferland for technical assistance. We co-authors were all at Brown University when this work was developed and we remain Brown Alumni. We are grateful to Brown University for the extraordinary opportunity afforded us to work together: Bearer as tenured professor and PI, DeGiorgis as PhD candidate, and Jang in the BA-MD program. This work was funded by Frederick Bang Summer Whitman Fellowships from MBL (E.L.B.), the Dart Foundation (E.L.B.) as well as NIGMS RO1 GM47368, NINDS RO1 NS046810 and RO1 NS062184 (E.L.B.), and the National Science Foundation under EPSCoR Cooperative Agreement #OIA-1655221 (J.A.D.). | ||||||||||||||
| Funders: |
| ||||||||||||||
| Subject Keywords: | Axonal transport, Intracellular transport, Kinesin, Amyloid precursor protein, Microtubule-based transport, Herpes simplex virus type 1, Doryteuthis (Loligo) pealei, Giant axon, Cytoskeleton | ||||||||||||||
| Series Name: | Methods in Molecular Biology | ||||||||||||||
| Issue or Number: | 2431 | ||||||||||||||
| DOI: | 10.1007/978-1-0716-1990-2_1 | ||||||||||||||
| Record Number: | CaltechAUTHORS:20220511-183651271 | ||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220511-183651271 | ||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
| ID Code: | 114678 | ||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||||
| Deposited On: | 11 May 2022 21:18 | ||||||||||||||
| Last Modified: | 11 May 2022 21:18 |
Repository Staff Only: item control page
