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Magnetic-Powered Janus Cell Robots Loaded with Oncolytic Adenovirus for Active and Targeted Virotherapy of Bladder Cancer

Cong, Zhaoqing and Tang, Songsong and Xie, Leiming and Yang, Ming and Li, Yangyang and Lu, Dongdong and Li, Jiahong and Yang, Qingxin and Chen, Qiwei and Zhang, Zhiqiang and Zhang, Xueji and Wu, Song (2022) Magnetic-Powered Janus Cell Robots Loaded with Oncolytic Adenovirus for Active and Targeted Virotherapy of Bladder Cancer. Advanced Materials, 34 (26). Art. No. 2201042. ISSN 0935-9648. doi:10.1002/adma.202201042. https://resolver.caltech.edu/CaltechAUTHORS:20220520-388218000

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Abstract

A unique robotic medical platform is designed by utilizing cell robots as the active “Trojan horse” of oncolytic adenovirus (OA), capable of tumor-selective binding and killing. The OA-loaded cell robots are fabricated by entirely modifying OA-infected 293T cells with cyclic arginine–glycine–aspartic acid tripeptide (cRGD) to specifically bind with bladder cancer cells, followed by asymmetric immobilization of Fe₃O₄ nanoparticles (NPs) on the cell surface. OA can replicate in host cells and induce cytolysis to release the virus progeny to the surrounding tumor sites for sustainable infection and oncolysis. The asymmetric coating of magnetic NPs bestows the cell robots with effective movement in various media and wireless manipulation with directional migration in a microfluidic device and bladder mold under magnetic control, further enabling steerable movement and prolonged retention of cell robots in the mouse bladder. The biorecognition of cRGD and robust, controllable propulsion of cell robots work synergistically to greatly enhance their tissue penetration and anticancer efficacy in the 3D cancer spheroid and orthotopic mouse bladder tumor model. Overall, this study integrates cell-based microrobots with virotherapy to generate an attractive robotic system with tumor specificity, expanding the operation scope of cell robots in biomedical community.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/adma.202201042DOIArticle
ORCID:
AuthorORCID
Li, Jiahong0000-0001-7938-9589
Zhang, Xueji0000-0002-0035-3821
Wu, Song0000-0001-8859-7806
Additional Information:© 2022 Wiley-VCH GmbH. Issue Online: 01 July 2022; Version of Record online: 27 May 2022; Accepted manuscript online: 22 April 2022; Manuscript revised: 09 April 2022; Manuscript received: 31 January 2022. This work was supported by National Key Research and Development Program of China (Grant No. 2017YFA0105900), Guangdong Special Support Program of Youth Talent with Scientific and Technological Innovation, National Natural Science Foundation Fund of China (Grant Nos. 81922046, 61931024, 81802741, and 32101133), Special Funds for Strategic Emerging Industries Development in Shenzhen (Grant No. 20180309163446298), Shenzhen Science and Technology Innovation Commission (Grant No. RCJC20200714114557005), Shenzhen Science and Technology Program (Grant No. JCYJ20210324125006019), Shenzhen Key Laboratory Program (Grant No. ZDSYS20190902092857146), and China Postdoctoral Council International Postdoctoral Exchange Fellowship Program (Grant No. PC2021078). All animal experiments were strictly conducted in compliance with the guidelines of the Animal Use and Care Administrative Advisory Committee of Shenzhen Luohu Hospital Group. The authors declare no conflict of interest. Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Funders:
Funding AgencyGrant Number
National Key Research and Development Program of China2017YFA0105900
Guangdong Special Support Program of Youth Talent with Scientific and Technological InnovationUNSPECIFIED
National Natural Science Foundation of China81922046
National Natural Science Foundation of China61931024
National Natural Science Foundation of China81802741
National Natural Science Foundation of China32101133
Special Funds for Strategic Emerging Industries Development in Shenzhen20180309163446298
Shenzhen Science and Technology Innovation CommissionRCJC20200714114557005
Shenzhen Science and Technology ProgramJCYJ20210324125006019
Shenzhen Key LaboratoryZDSYS20190902092857146
China Postdoctoral Science FoundationPC2021078
Subject Keywords:bladder cancer; cell-based microrobots; magnetic propulsion; oncolytic adenovirus; virotherapy
Issue or Number:26
DOI:10.1002/adma.202201042
Record Number:CaltechAUTHORS:20220520-388218000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220520-388218000
Official Citation:Cong, Z., Tang, S., Xie, L., Yang, M., Li, Y., Lu, D., Li, J., Yang, Q., Chen, Q., Zhang, Z., Zhang, X., Wu, S., Magnetic-Powered Janus Cell Robots Loaded with Oncolytic Adenovirus for Active and Targeted Virotherapy of Bladder Cancer. Adv. Mater. 2022, 34, 2201042. https://doi.org/10.1002/adma.202201042
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:114836
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 May 2022 14:19
Last Modified:20 Jul 2022 17:13

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