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Cell growth dynamics in two types of apical meristems in fern gametophytes

Wu, Xiao and Yan, An and Yang, Xi and Banks, Jo Ann and Zhang, Shaoling and Zhou, Yun (2022) Cell growth dynamics in two types of apical meristems in fern gametophytes. Plant Journal, 111 (1). pp. 149-163. ISSN 0960-7412. doi:10.1111/tpj.15784. https://resolver.caltech.edu/CaltechAUTHORS:20220621-137619700

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Abstract

In contrast to seed plants, the gametophytes of seed-free plants develop pluripotent meristems, which promote and sustain their independent growth and development. To date, the cellular basis of meristem development in gametophytes of seed-free ferns remains largely unknown. In this study, we used Woodsia obtusa, the blunt-lobe cliff fern, to quantitatively determine cell growth dynamics in two different types of apical meristems – the apical initial centered meristem and the multicellular apical meristem in gametophytes. Through confocal time-lapse live imaging and computational image analysis and quantification, we determined unique patterns of cell division and growth that sustain or terminate apical initials, dictate the transition from apical initials to multicellular apical meristems, and drive proliferation of apical meristems in ferns. Quantitative results showed that small cells correlated to active cell division in fern gametophytes. The marginal cells of multicellular apical meristems in fern gametophytes undergo division in both anticlinal and periclinal orientations, not only increasing cell numbers but also playing a dominant role in increasing cell layers during gametophyte development. All these findings provide insights into the function and regulation of meristems in gametophytes of seed-free vascular plants, suggesting both conserved and diversified mechanisms underlying meristem cell proliferation across land plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1111/tpj.15784DOIArticle
ORCID:
AuthorORCID
Zhang, Shaoling0000-0002-1835-1824
Zhou, Yun0000-0003-4105-5174
Additional Information:© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Issue Online: 05 July 2022; Version of Record online: 12 May 2022; Accepted manuscript online: 21 April 2022; Manuscript accepted: 19 April 2022; Manuscript revised:11 April 2022; Manuscript received: 08 November 2021. The authors thank the American Fern Society, Brian Aikins, and Gordon McNickle for sharing the spores and the Purdue Bindley Bioscience Facility for access to the ZEISS LSM880 confocal microscope. The authors also thank Dr. Elliot Meyerowitz from Caltech for the support and encouragements. This work was supported by Purdue University start-up funds (to YZ), the grant from Purdue Center for Plant Biology (to YZ), and the NSF IOS-1931114 grant (to YZ and JB). Author Contributions: YZ conceived the research; XW and XY performed the experiments; JAB and YZ supervised the experiments at Purdue; XW, XY, JAB, SZ, and YZ discussed the experimental results; AY, XW, and YZ performed computational analysis and quantification; XW and YZ wrote the manuscript; and JAB, AY, and SZ revised the manuscript. All authors approved the manuscript. The authors declare no conflict of interest. Data Availability Statement: All the data were included in the manuscript and the supplementary files.
Funders:
Funding AgencyGrant Number
Purdue UniversityUNSPECIFIED
NSFIOS-1931114
Subject Keywords:cell division, ferns, apical cell, apical meristem, gametophytes, cell size, Woodsia obtusa
Issue or Number:1
DOI:10.1111/tpj.15784
Record Number:CaltechAUTHORS:20220621-137619700
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220621-137619700
Official Citation:Wu, X., Yan, A., Yang, X., Banks, J.A., Zhang, S. and Zhou, Y. (2022), Cell growth dynamics in two types of apical meristems in fern gametophytes. Plant J, 111: 149-163. https://doi.org/10.1111/tpj.15784
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:115215
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jun 2022 22:27
Last Modified:15 Jul 2022 22:22

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