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Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes

Geng, Yuan and Yan, An and Zhou, Yun (2022) Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes. Communications Biology, 5 . Art. No. 650. ISSN 2399-3642. PMCID PMC9249879. doi:10.1038/s42003-022-03627-y. https://resolver.caltech.edu/CaltechAUTHORS:20220705-671503000

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Abstract

Fern gametophytes are autotrophic and independent of sporophytes, and they develop pluripotent meristems that drive prothallus development and sexual reproduction. To reveal cellular dynamics during meristem development in fern gametophytes, we performed long-term time-lapse imaging and determined the real-time lineage, identity and division activity of each single cell from meristem initiation to establishment in gametophytes of the fern Ceratopteris richardii. Our results demonstrate that in Ceratopteris gametophytes, only a few cell lineages originated from the marginal layer contribute to meristem initiation and proliferation, and the meristem lacks a distinguishable central zone or apical cell with low division activity. Within the meristem, cell division is independent of cell lineages and cells at the marginal layer are more actively dividing than inner cells. Furthermore, the meristem triggers differentiation of adjacent cells into egg-producing archegonia in a position-dependent manner. These findings advance the understanding of diversified meristem and gametophyte development in land plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s42003-022-03627-yDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249879/PubMed CentralArticle
ORCID:
AuthorORCID
Yan, An0000-0002-4676-5442
Zhou, Yun0000-0003-4105-5174
Additional Information:© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received 08 February 2022. Accepted 22 June 2022. Published 01 July 2022. The authors thank Purdue Bindley Bioscience Facility for the access of the ZEISS LSM880 confocal microscope and thank Dr. Jody Banks for helpful comments and suggestions. Authors also thank Dr. Elliot Meyerowitz from Caltech for the support and encouragement. This work was supported by Purdue University start-up and funds from Purdue Center for Plant Biology (to Y.Z.) and by the NSF IOS 1931114 grant (to Y.Z.). Data availability. The data that support the results and conclusions of this study are available within the paper, Supplementary Information and Supplementary Data 1–5. DNA sequence of the expression cassette for the pCrHAM::H2B-GFP::3’CrHAM reporter was deposited in NCBI with the accession number ON787967 and is also shown in Supplementary Fig. 18. Any other supporting information is available from the corresponding author upon request. Code availability. The code is available from the corresponding author upon request. Contributions. Y.G. and Y.Z. conceived the research direction; Y.G. and Y.Z. discussed and interpreted results; Y.G. and Y.Z. performed experiments; Y.Z. supervised the research progress; A.Y. and Y.Z. performed image analysis; A.Y., Y.Z., and Y.G. performed analyses of cell lineages and cell divisions; Y.G. and Y.Z. wrote the manuscript; and A.Y. revised the manuscript. The authors declare no competing interests. Peer review information. Communications Biology thanks the anonymous reviewers for their contribution to the peer review of this work. Primary Handling Editors: Manuel Breuer and Simona Chera.
Funders:
Funding AgencyGrant Number
Purdue UniversityUNSPECIFIED
NSFIOS-1931114
PubMed Central ID:PMC9249879
DOI:10.1038/s42003-022-03627-y
Record Number:CaltechAUTHORS:20220705-671503000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220705-671503000
Official Citation:Geng, Y., Yan, A. & Zhou, Y. Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes. Commun Biol 5, 650 (2022). https://doi.org/10.1038/s42003-022-03627-y
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:115305
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Jul 2022 22:17
Last Modified:08 Jul 2022 22:17

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