Rubanov, Moshe and Dorsey, Phillip and Scalise, Dominic and Schulman, Rebecca (2022) Sequential Activation of Spatially Localized Oligonucleotides. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220705-346751000
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20220705-346751000
Abstract
The programmable spatiotemporal activation of molecules could be used to create stimuli-responsive biomaterials capable of executing multistage sensing and computational processes. Here we develop a method for the activation of different DNA molecules in precisely specified locations and times within hydrogels. The activation locations are determined by patterning molecules to be released in their inactive form within hydrogels at resolutions of tens of microns, whereas the time of activation is controlled by a DNA strand displacement reaction cascade that releases the target DNA oligonucleotides at precisely specified time intervals. This programmed activation of DNA from hydrogel substrates thus enables the scalable development of DNA-based reaction-diffusion systems that regulate DNA strand availability in space and time. This system could be used as a platform to autonomously activate predefined chemical signals embedded within a soft material.
Item Type: | Report or Paper (Discussion Paper) | ||||||||||||
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Additional Information: | The content is available under CC BY NC ND 4.0 License. The authors wish to thank Samuel Schaffter, Ruohong Shi and Wenlu Wang for helpful advice and discussions. This work was supported by DOE DE-SC0010426, with additional support from ARO W911NF2010057, DOE BES 221874, a JHU Catalyst Award and NSF SHF-1527377 to R.S. M.R. was supported by DOE DE-SC0010426 and ARO W911NF2010057, P.D. by the JHU Catalyst Award and DOE BES 221874, and D.S. by NSF SHF-1527377. Author Contributions. M.R., R.S. and P.D. wrote the paper. D.S. and M.R. conceived of the idea. M.R. and P.D. designed the experiments, M.R. performed the experiments. All authors approved the final version of the manuscript. The author(s) have declared they have no conflict of interest with regard to this content. | ||||||||||||
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Subject Keywords: | DNA nanotechnology, DNA-functionalized hydrogels, DNA strand-displacement, reaction networks, maskless photolithography | ||||||||||||
DOI: | 10.26434/chemrxiv-2022-4jcv6 | ||||||||||||
Record Number: | CaltechAUTHORS:20220705-346751000 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220705-346751000 | ||||||||||||
Official Citation: | Rubanov M, Dorsey P, Scalise D, Schulman R. Sequential Activation of Spatially Localized Oligonucleotides. ChemRxiv. 2022. doi:10.26434/chemrxiv-2022-4jcv6 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 115344 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | George Porter | ||||||||||||
Deposited On: | 08 Jul 2022 22:47 | ||||||||||||
Last Modified: | 25 Jul 2022 23:13 |
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