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A fluorometric method for the estimation of tryptophan

Gordon, Malcolm and Mitchell, Herschel K. (1949) A fluorometric method for the estimation of tryptophan. Journal of Biological Chemistry, 180 (3). pp. 1065-1070. ISSN 0021-9258.

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The various colorimetric methods now employed for the estimation of tryptophan are not specific for tryptophan but give colored products with many compounds containing the indole nucleus, including indole itself. In order to facilitate studies of the enzymatic synthesis of tryptophan from indole and serine by extracts of Neurospora (1), a search for a rapid, quantitative method for the estimation of tryptophan in the presence of indole was undertaken. Tauber (2) reported that tryptophan gives a green fluorescence when treated with 70 to 72 per cent perchloric acid at room temperature. Modification of this procedure has led to a method for the rapid estimation of tryptophan, without preliminary extraction of indole, in hydrolyzed or unhydrolyzed tryptophan-containing materials.

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Additional Information:© 1949 by the American Society for Biochemistry and Molecular Biology. Received for publication, May 7, 1949. This work was supported by funds from the Rockefeller Foundation and by funds from the Atomic Energy Commission, administered through contract with the Office of Naval Research, United States Navy (contract N-6-onr-244, Task Order 5). [M.G. was a] Merck Fellow of the National Research Council.
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Rockefeller FoundationUNSPECIFIED
Atomic Energy CommissionUNSPECIFIED
Office of Naval ResearchN-6-onr-244, Task Order 5
National Research CouncilUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:GORjbc49
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11557
Deposited By: Tony Diaz
Deposited On:12 Sep 2008 01:17
Last Modified:03 Oct 2019 00:20

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