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Fate Bias and Transcriptional Memory of human B cells

Swift, Michael and Horns, Felix and Quake, Stephen R. (2022) Fate Bias and Transcriptional Memory of human B cells. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220720-917058000

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Abstract

Lineage tracking offers a direct approach to study cell fate determination. In this work we combined single cell transcriptomics and lineage tracing to better understand fate-choice in human B cells. Using antibody sequences to trace cell lineage during in vitro differentiation, we identified intrinsic proliferative and cell fate biases of B cell subtypes. Clonal analysis revealed that IgM memory B cells were more proliferative than any other B cell subtype, and that cells from the same clone had highly concordant fates. We found that transcriptional memory within clones varies across genes, with strongest persistence in genes related to cell fate determination. Similar persistent transcriptional programs were observed in human plasma cells from bone marrow, suggesting that these programs maintain long-term cell fate in vivo. These results show that cell-intrinsic fate bias influences human B cell differentiation and reveal molecular programs underpinning cell fate determination in B cells.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2022.07.14.499766DOIDiscussion Paper
https://www.biorxiv.org/content/10.1101/2022.07.14.499766v1.supplementary-materialPublisherSupporting Information
https://github.com/michael-swift/seqclone3Related ItemCode
ORCID:
AuthorORCID
Swift, Michael0000-0001-6391-0050
Quake, Stephen R.0000-0002-1613-0809
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. We thank Ivana Cvijovic, Elisabeth Jerison, Derek Croote, Bali Pulendran, Dan Jarosz, and Daria-Mochly Rosen for useful discussions and helpful comments on the manuscript. This work was supported by the National Science Foundation Graduate Research Fellowship Program (to M.A.S.) The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipUNSPECIFIED
DOI:10.1101/2022.07.14.499766
Record Number:CaltechAUTHORS:20220720-917058000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220720-917058000
Official Citation:Fate Bias and Transcriptional Memory of human B cells Michael Swift, Felix Horns, Stephen R. Quake bioRxiv 2022.07.14.499766; doi: https://doi.org/10.1101/2022.07.14.499766
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:115691
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Jul 2022 22:34
Last Modified:21 Jul 2022 22:34

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