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Control of protein activity by photoinduced spin polarized charge reorganization

Ghosh, Shirsendu and Banerjee Ghosh, Koyel and Levy, Dorit and Scheerer, David and Riven, Inbal and Shin, Jieun and Gray, Harry B. and Naaman, Ron and Haran, Gilad (2021) Control of protein activity by photoinduced spin polarized charge reorganization. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20220720-917086000

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Abstract

Considerable electric fields are present within living cells, and the role of bioelectricity has been well established at the organismal level. Yet little is known about electric-field effects on protein function. Here we use phototriggered charge injection from a site-specifically attached ruthenium photosensitizer to directly demonstrate the effects of charge redistribution within a protein. We find that binding of an antibody to phosphoglycerate kinase (PGK) is increased two folds under illumination. Remarkably, illumination is found to suppress the enzymatic activity of PGK by a factor as large as three. These responses are sensitive to the photosensitizer position on the protein. Surprisingly, left (but not right) circularly polarized light elicits these responses, indicating that the electrons involved in the observed dynamics are spin polarized, due to spin filtration by protein chiral structures. Our results directly establish the contribution of electrical polarization as an allosteric signal within proteins. Future experiments with phototriggered charge injection will allow delineation of charge rearrangement pathways within proteins and will further depict their effects on protein function.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.10.12.464058DOIDiscussion Paper
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Naaman, Ron0000-0003-1910-366X
Haran, Gilad0000-0003-1837-9779
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. S.G. and K.B.-G. contributed equally to this work. This work was partially supported by a grant to G.H. from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 742637, SMALLOSTERY), a grant from the Israel Science Foundation (no. 1250/19) and a grant from the Weizmann SABRA - Yeda-Sela – WRC program. G.H. holds the Hilda Pomeraniec Memorial Professorial Chair. R.N. acknowledges partial support from the MINERVA Foundation and from the Israel Ministry of Science and Technology. Work at Caltech was supported by the United States NIH (R01 DK019038 to H.B.G.). Author Contributions: S.G. and K.B.-G. designed the project, performed all experiments and analyzed the results, D.L. prepared labeled proteins, D.S. instructed and participated in enzyme kinetics experiments, J.S. and H.B.G provided materials, G.H. and R.N. conceived and supervised the research and wrote the paper with help from all authors. The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
European Research Council (ERC)742637
Israel Science Foundation1250/19
Weizmann SABRA - Yeda-Sela – WRC programUNSPECIFIED
Hilda Pomeraniec Memorial Professorial ChairUNSPECIFIED
MINERVA (Israel)UNSPECIFIED
Ministry of Science and Technology (Israel)UNSPECIFIED
NIHR01 DK019038
DOI:10.1101/2021.10.12.464058
Record Number:CaltechAUTHORS:20220720-917086000
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220720-917086000
Official Citation:Control of protein activity by photoinduced spin polarized charge reorganization Shirsendu Ghosh, Koyel Banerjee Ghosh, Dorit Levy, David Scheerer, Inbal Riven, Jieun Shin, Harry Gray, Ron Naaman, Gilad Haran bioRxiv 2021.10.12.464058; doi: https://doi.org/10.1101/2021.10.12.464058
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:115692
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Jul 2022 14:45
Last Modified:22 Jul 2022 14:45

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