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Bringing Vibrational Imaging to Chemical Biology with Molecular Probes

Du, Jiajun and Wang, Haomin and Wei, Lu (2022) Bringing Vibrational Imaging to Chemical Biology with Molecular Probes. ACS Chemical Biology, 17 (7). pp. 1621-1637. ISSN 1554-8929. doi:10.1021/acschembio.2c00200.

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As an emerging optical imaging modality, stimulated Raman scattering (SRS) microscopy provides invaluable opportunities for chemical biology studies using its rich chemical information. Through rapid progress over the past decade, the development of Raman probes harnessing the chemical biology toolbox has proven to play a key role in advancing SRS microscopy and expanding biological applications. In this perspective, we first discuss the development of biorthogonal SRS imaging using small tagging of triple bonds or isotopes and highlight their unique advantages for metabolic pathway analysis and microbiology investigations. Potential opportunities for chemical biology studies integrating small tagging with SRS imaging are also proposed. We next summarize the current designs of highly sensitive and super-multiplexed SRS probes, as well as provide future directions and considerations for next-generation functional probe design. These rationally designed SRS probes are envisioned to bridge the gap between SRS microscopy and chemical biology research and should benefit their mutual development.

Item Type:Article
Related URLs:
URLURL TypeDescription
Du, Jiajun0000-0003-2693-834X
Wang, Haomin0000-0001-7193-8651
Wei, Lu0000-0001-9170-2283
Additional Information:© 2022 American Chemical Society. Published online 30 June 2022. Published in issue 15 July 2022. L. Wei acknowledges startup funds from California Institute of Technology and Grant No. DP2 GM140919 from the National Institutes of Health. We thank A. Colazo for helpful discussion. The authors declare no competing financial interest.
Funding AgencyGrant Number
NIHDP2 GM140919
Subject Keywords:Biological imaging, Hydrocarbons, Labeling, Lipids, Probes
Issue or Number:7
Record Number:CaltechAUTHORS:20220721-8927000
Persistent URL:
Official Citation:Bringing Vibrational Imaging to Chemical Biology with Molecular Probes Jiajun Du, Haomin Wang, and Lu Wei ACS Chemical Biology 2022 17 (7), 1621-1637 DOI: 10.1021/acschembio.2c00200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:115746
Deposited By: George Porter
Deposited On:22 Jul 2022 21:48
Last Modified:22 Jul 2022 21:48

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