A Caltech Library Service

Identification of a component of Drosophila polar granules

Hay, Bruce A. and Ackerman, Larry and Barbel, Sandra and Jan, Lily Y. and Jan, Yuh Nung (1988) Identification of a component of Drosophila polar granules. Development, 103 (4). pp. 625-640. ISSN 0950-1991.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Information necessary for the formation of pole cells, precursors of the germ line, is provided maternally and localized to the posterior pole of the Drosophila egg. The maternal origin and posterior localization of polar granules suggest that they may be associated with pole cell determinants. We have generated an antibody (Mab46F11) against polar granules. In oocytes and early embryos, the Mab46F11 antigen is sharply localized to the posterior embryonic pole. In pole cells, it becomes associated with nuclear bodies within, and nuage around, the nucleus. Immunoreactivity remains associated with cells of the germ line throughout the life cycle of both males and females. This antibody recognizes a 72-74 X 10^(3) Mr protein and is useful both as a pole lineage marker and in biochemical studies of polar granules.

Item Type:Article
Related URLs:
URLURL TypeDescription
Hay, Bruce A.0000-0002-5486-0482
Additional Information:© 1988 by Company of Biologists. (Accepted 11 April 1988) We would like to thank T. Schüpbach and E. Wieschaus for sending us the vasaPD23 stock, and R. Lehmann and C. Nusslein-Volhard for sending us the pumillio680 stock. We also thank T. Kornberg for use of his fly cage facility. We especially thank K. Logan for help analysing the data concerning pole cell number, E. Bier, K. Logan, K. Neugebauer, and T. Schwarz for their valuable comments on the manuscript and Ms L. Schulte for help in preparing the manuscript. This work was supported by NTH and HHMI grants to L.Y.J. and Y.N.J. B.H. was supported by an NLH predoctoral training gTant. L.Y.J. and Y.N.J. are HHMI investigators.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Drosophila, polar granules, germ line, cytoplasmic determinants, monoclonal antibody
Issue or Number:4
Record Number:CaltechAUTHORS:HAYdev88
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11587
Deposited By: Archive Administrator
Deposited On:08 Sep 2008 23:53
Last Modified:02 Jul 2020 21:04

Repository Staff Only: item control page