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Essential role for PDGF signaling in ophthalmic trigeminal placode induction

McCabe, Kathryn L. and Bronner-Fraser, Marianne (2008) Essential role for PDGF signaling in ophthalmic trigeminal placode induction. Development, 135 (10). pp. 1863-1874. ISSN 0950-1991. doi:10.1242/dev.017954.

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Much of the peripheral nervous system of the head is derived from ectodermal thickenings, called placodes, that delaminate or invaginate to form cranial ganglia and sense organs. The trigeminal ganglion, which arises lateral to the midbrain, forms via interactions between the neural tube and adjacent ectoderm. This induction triggers expression of Pax3, ingression of placode cells and their differentiation into neurons. However, the molecular nature of the underlying signals remains unknown. Here, we investigate the role of PDGF signaling in ophthalmic trigeminal placode induction. By in situ hybridization, PDGF receptor β is expressed in the cranial ectoderm at the time of trigeminal placode formation, with the ligand PDGFD expressed in the midbrain neural folds. Blocking PDGF signaling in vitro results in a dose-dependent abrogation of Pax3 expression in recombinants of quail ectoderm with chick neural tube that recapitulate placode induction. In ovo microinjection of PDGF inhibitor causes a similar loss of Pax3 as well as the later placodal marker, CD151, and failure of neuronal differentiation. Conversely, microinjection of exogenous PDGFD increases the number of Pax3+ cells in the trigeminal placode and neurons in the condensing ganglia. Our results provide the first evidence for a signaling pathway involved in ophthalmic (opV) trigeminal placode induction.

Item Type:Article
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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© The Company of Biologists Ltd 2008. Accepted 20 March 2008. We thank Samuel Ki and Matthew Jones for technical support, Dr Peter Lwigale for sharing unpublished data, and Drs Sujata Bhattacharyya and Laura Gammill for critical reading of the manuscript. This work was funded by NIH R01 DE16459.
Funding AgencyGrant Number
NIHR01 DE16459
Subject Keywords:PDGF, Induction, Neurogenesis, Trigeminal placode, Chicken
Issue or Number:10
Record Number:CaltechAUTHORS:MCCdev08
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11594
Deposited By: Archive Administrator
Deposited On:09 Sep 2008 04:43
Last Modified:08 Nov 2021 22:00

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