A Caltech Library Service

A high-speed, modular display system for diverse neuroscience applications

Isaacson, Matthew and Ferguson, Lisa and Loesche, Frank and Ganguly, Ishani and Chen, Jim and Chiu, Andy and Liu, Jinyang and Dickson, William and Reiser, Michael (2022) A high-speed, modular display system for diverse neuroscience applications. . (Unpublished)

[img] PDF - Submitted Version
Creative Commons Attribution.

[img] Video (QuickTime) (Movie S1) - Supplemental Material
Creative Commons Attribution.

[img] Video (QuickTime) (Movie S2) - Supplemental Material
Creative Commons Attribution.

[img] Video (QuickTime) (Movie S3) - Supplemental Material
Creative Commons Attribution.

[img] Video (QuickTime) (Movie S4) - Supplemental Material
Creative Commons Attribution.


Use this Persistent URL to link to this item:


Visual stimulation of animals in the laboratory is a powerful technique for studying sensory control of complex behaviors. Since commercial displays are optimized for human vision, we established a novel display system based on custom-built modular LED panels that provides millisecond refresh, precise synchronization, customizable color combinations, and varied display configurations. This system simplifies challenging experiments. With variants of this display, we probed the speed limits of motion vision and examined the role of color vision in behavioral experiments with tethered flying Drosophila. Using 2-photon calcium imaging, we comprehensively mapped the tuning of visual projection neurons across the fly's field of view. Finally, using real-time behavior analysis, we developed low-latency interactive virtual environments and found that flying flies can independently control their navigation along two dimensions. This display system uniquely addresses most technical challenges of small animal vision experiments and is thoroughly documented for replicability.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper Information ItemCode
Isaacson, Matthew0000-0001-8797-0090
Loesche, Frank0000-0002-7780-6438
Reiser, Michael0000-0002-4108-4517
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. We thank Igor Negrashov and Bill Biddle for help in designing and fabricating the conical head mount, and Jon Arnold for designing the motorized rotational stage. We thank Aljsoscha Nern for the LPC1 split-GAL4 line and Edward Rogers for help with fly husbandry. We thank Ofer Mazor and Pavel Gorelik for feedback and updates to the documentation and code for the project. We are grateful to members of the Reiser lab, especially Eyal Gruntman, for feedback on the design of the system and discussions on the manuscript. This project was supported by the Howard Hughes Medical Institute. This article is subject to HHMI’s Open Access to Publications policy. HHMI lab heads have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication. The authors have declared no competing interest.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20220803-536045000
Persistent URL:
Official Citation:A high-speed, modular display system for diverse neuroscience applications Matthew Isaacson, Lisa Ferguson, Frank Loesche, Ishani Ganguly, Jim Chen, Andy Chiu, Jinyang Liu, William Dickson, Michael Reiser bioRxiv 2022.08.02.502550; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:116076
Deposited By: George Porter
Deposited On:04 Aug 2022 18:16
Last Modified:04 Aug 2022 18:16

Repository Staff Only: item control page