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Independent lines of evidence suggesting a major gap junctional protein with a molecular weight of 26,000

Finbow, Malcolm and Yancey, S. Barbara and Johnson, Ross and Revel, Jean-Paul (1980) Independent lines of evidence suggesting a major gap junctional protein with a molecular weight of 26,000. Proceedings of the National Academy of Sciences of the United States of America, 77 (2). pp. 970-974. ISSN 0027-8424.

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Several polypeptides have been described in the past as components of gap junction fractions. Of these, a peptide of M(r) 26,000 is found in gap junctions isolated from livers of different species under conditions that minimize proteolysis. Tryptic digestion of purified "intact" junctions causes the rapid disappearance of this peptide with a concomitant appearance of a band of M(r) 10,000, which has previously been found to be characteristic of junctional fractions isolated with the aid of proteolytic treatment. Both the peptides of M(r) 26,000 and 10,000 are missing from gap junction preparations after partial hepatectomy, when gap junctions are absent from the surface of the hepatocytes. They have reappeared in fractions from the livers of animals killed 3 days postoperatively, when gap junctions are again present in vivo.

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Additional Information:Copyright © 1980 by the National Academy of Sciences. Communicated by Don W. Fawcett, October 22, 1979. We thank Richard Gomer and Bruce Nicholson for their cooperation. The technical help of David Easter and Pat Koen was much appreciated. The research was supported by Research Grant GM 06965, Biomedical Research Support Program RR 07003, and Fellowship AM 05700 all from the National Institutes of Health (S.B.Y.), as well as support from the Northwest Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:polyacrylamide gels, membrane proteins, liver, regenerating liver
Issue or Number:2
Record Number:CaltechAUTHORS:FINpnas80
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1163
Deposited By: Tony Diaz
Deposited On:30 Dec 2005
Last Modified:02 Oct 2019 22:40

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