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Long-Duration and Non-Invasive Photoacoustic Imaging of Multiple Anatomical Structures in a Live Mouse Using a Single Contrast Agent

Khadria, Anjul and Paavola, Chad D. and Zhang, Yang and Davis, Samuel P. X. and Grealish, Patrick F. and Maslov, Konstantin and Shi, Junhui and Beals, John M. and Oladipupo, Sunday S. and Wang, Lihong V. (2022) Long-Duration and Non-Invasive Photoacoustic Imaging of Multiple Anatomical Structures in a Live Mouse Using a Single Contrast Agent. Advanced Science, 9 (28). Art. No. 2202907. ISSN 2198-3844. doi:10.1002/advs.202202907. (In Press)

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Long-duration in vivo simultaneous imaging of multiple anatomical structures is useful for understanding physiological aspects of diseases, informative for molecular optimization in preclinical models, and has potential applications in surgical settings to improve clinical outcomes. Previous studies involving simultaneous imaging of multiple anatomical structures, for example, blood and lymphatic vessels as well as peripheral nerves and sebaceous glands, have used genetically engineered mice, which require expensive and time-consuming methods. Here, an IgG4 isotype control antibody is labeled with a near-infrared dye and injected into a mouse ear to enable simultaneous visualization of blood and lymphatic vessels, peripheral nerves, and sebaceous glands for up to 3 h using photoacoustic microscopy. For multiple anatomical structure imaging, peripheral nerves and sebaceous glands are imaged inside the injected dye-labeled antibody mass while the lymphatic vessels are visualized outside the mass. The efficacy of the contrast agent to label and localize deep medial lymphatic vessels and lymph nodes using photoacoustic computed tomography is demonstrated. The capability of a single injectable contrast agent to image multiple structures for several hours will potentially improve preclinical therapeutic optimization, shorten discovery timelines, and enable clinical treatments.

Item Type:Article
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URLURL TypeDescription CentralArticle ItemDiscussion Paper
Khadria, Anjul0000-0002-9771-3650
Paavola, Chad D.0000-0001-6952-1400
Zhang, Yang0000-0002-4168-9225
Maslov, Konstantin0000-0003-3408-8840
Shi, Junhui0000-0002-5741-2781
Oladipupo, Sunday S.0000-0001-6555-0693
Wang, Lihong V.0000-0001-9783-4383
Additional Information:The authors acknowledge Rui Cao, California Institute of Technology foruseful discussions. Research Funding: Eli Lilly and Company.
Funding AgencyGrant Number
Eli Lilly and CompanyUNSPECIFIED
Issue or Number:28
Record Number:CaltechAUTHORS:20220825-833128900.761
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:116444
Deposited By: Melissa Ray
Deposited On:31 Aug 2022 15:44
Last Modified:02 Mar 2023 21:54

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