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Preliminary Studies on the Use of Monoclonal Antibodies as Probes for Sympathetic Development

Chun, Linda L. Y. and Patterson, Paul H. and Cantor, Harvey (1980) Preliminary Studies on the Use of Monoclonal Antibodies as Probes for Sympathetic Development. Journal of Experimental Biology, 89 . pp. 73-83. ISSN 0022-0949.

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The precise structural organization and proper functioning of the adult nervous system depend on the ability of neurones to make highly ordered synaptic connexions. To define molecules involved in the development of these connexions and to study their functional roles, we use primary cultures of dissociated rat sympathetic neurones grown in the virtual absence of non-neuronal cells. These neurones can develop adrenergic or cholinergic properties, depending on the environment in which they are grown. This ability to manipulate neuronal phenotype is being used in an attempt to identify cell surface macromolecules that are important in the development or function of adrenergic and cholinergic properties. We have produced monoclonal antibodies against the surface membranes of these neurones and are in the process of characterizing them. Results are presented on the binding specificity of one of these antibodies and on the effect of two other antibodies on neurotransmitter synthesis, uptake, and release.

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Additional Information:Published by Company of Biologists 1980. We wish to thank D. McDowell and J. Smith for help with the culturing, T.L. Wang for technical assistance, P. Walicke and A. Doupe for supplying nerve growth factor, and J. Gagliardi and L. Corcoran for help with the manuscript. This research was supported by grants from the NINCDS and NIAID. L.L.Y.C. was a Fellow in Cancer Research supported by Grant DRG-148-F of the Damon Runyon-Walter Winchell Cancer Fund, P.H.P. is a Rita Allen Foundation Scholar, and H.C. is a Scholar of the Leukemia Society of America.
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National Institute of Neurological and Communicative Disorders and StrokeUNSPECIFIED
National Institute of Allergy and Infectious DiseasesUNSPECIFIED
Damon Runyan-Walter Winchell Cancer Research FundDRG-148-F
Rita Allen FoundationUNSPECIFIED
Leukemia Society of AmericaUNSPECIFIED
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ID Code:11656
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Deposited On:17 Sep 2008 06:00
Last Modified:03 Oct 2019 00:21

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