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Linear signaling in the Toll-Dorsal pathway of Drosophila: activated Pelle kinase specifies all threshold outputs of gene expression while the bHLH protein Twist specifies a subset

Stathopoulos, Angelike and Levine, Michael (2002) Linear signaling in the Toll-Dorsal pathway of Drosophila: activated Pelle kinase specifies all threshold outputs of gene expression while the bHLH protein Twist specifies a subset. Development, 129 (14). pp. 3411-3419. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:STAdev02

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Abstract

Differential activation of the Toll receptor leads to the formation of a broad Dorsal nuclear gradient that specifies at least three patterning thresholds of gene activity along the dorsoventral axis of precellular embryos. We investigate the activities of the Pelle kinase and Twist basic helix-loop-helix (bHLH) transcription factor in transducing Toll signaling. Pelle functions downstream of Toll to release Dorsal from the Cactus inhibitor. Twist is an immediate-early gene that is activated upon entry of Dorsal into nuclei. Transgenes misexpressing Pelle and Twist were introduced into different mutant backgrounds and the patterning activities were visualized using various target genes that respond to different thresholds of Toll-Dorsal signaling. These studies suggest that an anteroposterior gradient of Pelle kinase activity is sufficient to generate all known Toll-Dorsal patterning thresholds and that Twist can function as a gradient morphogen to establish at least two distinct dorsoventral patterning thresholds. We discuss how the Dorsal gradient system can be modified during metazoan evolution and conclude that Dorsal-Twist interactions are distinct from the interplay between Bicoid and Hunchback, which pattern the anteroposterior axis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/129/14/3411PublisherArticle
ORCID:
AuthorORCID
Stathopoulos, Angelike0000-0001-6597-2036
Additional Information:© 2002 The Company of Biologists Limited. Accepted 30 April 2002. We thank Tony Ip for providing snail mutant flies, Maria Leptin for sending a full-length twist cDNA plasmid, James Manley for providing the anti-Pelle antibody, and Steve Wasserman for sending Pelle-Tor and Pelle-Tor4021 plasmids. This work was funded by a grant from the NIH (GM46638) A. S. is supported by a postdoctoral fellowship from the NIH (GM20352).
Funders:
Funding AgencyGrant Number
NIHGM46638
NIHGM20352
Subject Keywords:Dorsoventral patterning, Drosophila embryo, Dorsal, Pelle, Twist, Gradient thresholds
Issue or Number:14
Record Number:CaltechAUTHORS:STAdev02
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:STAdev02
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11661
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:17 Sep 2008 20:12
Last Modified:03 Oct 2019 00:21

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