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High-throughput force measurement of individual kinesin-1 motors during multi-motor transport

Shukla, Saurabh and Troitskaia, Alice and Swarna, Nikhila and Maity, Barun Kumar and Tjioe, Marco and Bookwalter, Carol S. and Trybus, Kathleen M. and Chemla, Yann R. and Selvin, Paul R. (2022) High-throughput force measurement of individual kinesin-1 motors during multi-motor transport. Nanoscale, 14 (34). pp. 12463-12475. ISSN 2040-3364. doi:10.1039/d2nr01701f. https://resolver.caltech.edu/CaltechAUTHORS:20220901-157280800

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Abstract

Molecular motors often work in teams to move a cellular cargo. Yet measuring the forces exerted by each motor is challenging. Using a sensor made with denatured ssDNA and multi-color fluorescence, we measured picoNewtons of forces and nanometer distances exerted by individual constrained kinesin-1 motors acting together while driving a common microtubule in vitro. We find that kinesins primarily exerted less than 1 pN force, even while the microtubule is bypassing artificial obstacles of 20–100 nanometer size. Occasionally, individual forces increase upon encountering obstacles, although at other times they do not, with the cargo continuing in a directional manner. Our high-throughput technique, which can measure forces by many motors simultaneously, is expected to be useful for many different types of molecular motors.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/D2NR01701FDOIArticle
ORCID:
AuthorORCID
Shukla, Saurabh0000-0002-7632-8163
Maity, Barun Kumar0000-0002-0749-3405
Selvin, Paul R.0000-0002-3658-4218
Additional Information:This work was supported by the NIH grants R01 GM132392 (to P. R. S.), GM120353 (to Y. R. C.), R35 GM136288 (to K. M. T.) and by the NSF Physics Frontiers Center (PFC) grant PHY-1430124 ("Center for the Physics of Living Cells").
Funders:
Funding AgencyGrant Number
NIHR01 GM132392
NIHGM120353
NIHR35 GM136288
NSFPHY-1430124
Issue or Number:34
DOI:10.1039/d2nr01701f
Record Number:CaltechAUTHORS:20220901-157280800
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20220901-157280800
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:116698
Collection:CaltechAUTHORS
Deposited By: Melissa Ray
Deposited On:09 Sep 2022 16:45
Last Modified:09 Sep 2022 16:45

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