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Voltage-Dependent Hydration and Conduction Properties of the Hydrophobic Pore of the Mechanosensitive Channel of Small Conductance

Spronk, Steven A. and Elmore, Donald E. and Dougherty, Dennis A. (2006) Voltage-Dependent Hydration and Conduction Properties of the Hydrophobic Pore of the Mechanosensitive Channel of Small Conductance. Biophysical Journal, 90 (10). pp. 3555-3569. ISSN 0006-3495. PMCID PMC1440736. https://resolver.caltech.edu/CaltechAUTHORS:SPRObj06

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Abstract

A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydrophobic gating. This mechanism has been implicated in the gating of several channels, including the mechanosensitive channel of small conductance (MscS). Although the pore in the crystal structure of MscS is wide and was initially hypothesized to be open, it is lined by hydrophobic residues and may represent a nonconducting state. Molecular dynamics simulations were performed on MscS to determine whether or not the structure can conduct ions. Unlike previous simulations of hydrophobic nanopores, electric fields were applied to this system to model the transmembrane potential, which proved to be important. Although simulations without a potential resulted in a dehydrated, occluded pore, the application of a potential increased the hydration of the pore and resulted in current flow through the channel. The calculated channel conductance was in good agreement with experiment. Therefore, it is likely that the MscS crystal structure is closer to a conducting than a nonconducting state.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1529/biophysj.105.080432DOIArticle
http://www.biophysj.org/cgi/content/abstract/90/10/3555PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1440736/PubMed CentralArticle
ORCID:
AuthorORCID
Dougherty, Dennis A.0000-0003-1464-2461
Contact Email Address:dadougherty@caltech.edu
Additional Information:© 2006 The Biophysical Society. Submitted December 26, 2005, and accepted for publication February 1, 2006. Originally published as Biophys J. BioFAST on February 24, 2006. doi:10.1529/biophysj.105.080432 This work was supported by a National Institutes of Health program project grant, GM 62532.
Funders:
Funding AgencyGrant Number
NIHGM 62532
Issue or Number:10
PubMed Central ID:PMC1440736
Record Number:CaltechAUTHORS:SPRObj06
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:SPRObj06
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11670
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:18 Sep 2008 03:57
Last Modified:03 Oct 2019 00:21

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