Feuda, Roberto (2022) The temporal dynamics of the sea urchin regulome. Biology Open, 11 (9). Art. no. bio059216. ISSN 2046-6390. PMCID PMC9493716. doi:10.1242/bio.059216. https://resolver.caltech.edu/CaltechAUTHORS:20220916-665766000
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Abstract
In this work, we used Nanostring N-counter technology, to evaluate the mRNA expression level of more than 330 regulatory genes over 34 time points covering the first three days of development of the sea urchin larvae. The hierarchical clustering of the mRNAs expression levels has identified groups corresponding to the major developmental landmarks (e.g. maternal to zygotic transition and gastrulation). Furthermore, comparison with previous experiments indicates high reproducibility of mRNA level temporal dynamics across batches. Finally, we generated an online tool to visualise gene expression during sea urchin larval development. The site can be accessed at and https://www621.lamp.le.ac.uk/nanostring_app/nanostring/.
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| Additional Information: | I would like to thank Eric Davidson and Isabelle Peter for their tireless support. Johnathan Valencia and Enhu Li for the help in embryos collections. Julius Barsi for designing the Codeset and Maryam Ghaffary Saadat for developing the visualisation tool. Ezio Rosato, Alessandra Perugini and the Feuda lab for comments on the MS. This work is partially supported by a Royal Society University Research Fellowship (UF160226) and a Royal Society Research grant (RGF\EA\201005) to R.F. | |||||||||
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| Issue or Number: | 9 | |||||||||
| PubMed Central ID: | PMC9493716 | |||||||||
| DOI: | 10.1242/bio.059216 | |||||||||
| Record Number: | CaltechAUTHORS:20220916-665766000 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20220916-665766000 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 117021 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Olivia Warschaw | |||||||||
| Deposited On: | 29 Oct 2022 22:15 | |||||||||
| Last Modified: | 01 Nov 2022 18:11 |
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