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A cell-specific enhancer that specifies lin-3 expression in the C. elegans anchor cell for vulval development

Hwang, Byung Joon and Sternberg, Paul W. (2004) A cell-specific enhancer that specifies lin-3 expression in the C. elegans anchor cell for vulval development. Development, 131 (1). pp. 143-151. ISSN 0950-1991. doi:10.1242/dev.00924.

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During C. elegans vulval development, the anchor cell (AC) in the somatic gonad expresses lin-3, activating the EGF receptor signaling pathway in vulval precursor cells (VPCs) and thereby inducing and patterning VPCs. Previous studies with lin-3 mutants and transgene expression have revealed that the level of LIN-3 in the AC must be precisely regulated for proper vulval development. To understand how lin-3 expression is achieved in the AC, we identified a 59 bp lin-3 enhancer sufficient to activate lin-3 transcription solely in the AC. The enhancer contains two E-box elements, and one FTZ-F1 nuclear hormone receptor (NHR) binding site that is mutated in a vulvaless mutant, lin-3(e1417). Mutagenesis studies show that both E-boxes and the NHR binding site are necessary to express lin-3 in the AC. In vitro DNA-binding studies and in vivo functional assays indicate that distinct trans-acting factors, including the E-protein/Daughterless homolog HLH-2 and unidentified nuclear hormone receptor(s), are necessary for lin-3 transcription in the AC and thus are involved in vulval development.

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Sternberg, Paul W.0000-0002-7699-0173
Additional Information:Published by The Company of Biologists 2004. Accepted 23 September 2003. We are grateful to X. Karp and I. Greenwald for communicating unpublished data on hlh-2; to Y Kohara for nhr-25 cDNA; and to A. Fire for gfp constructs. We thank J. Lee and H. Y. Yoon in W. Dunphy's laboratory for helping with phosphoimager analysis and expressing proteins in insect cells. Great appreciation for carefully reading the manuscript is given to C. Bastiani, B. Gupta, T. Inoue, Y. Kee, J. S. Kim, N. Moghal and S. Vernooy. This work was supported by the Howard Hughes Medical Institute with which P.W.S. is an investigator and B.J.H. was an associate.
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Howard Hughes Medical InstituteUNSPECIFIED
Subject Keywords:EGF, LIN-3, HLH-2, E-protein/Daughterless, Nuclear hormone receptor, Anchor cell, Vulval induction
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ID Code:11718
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Deposited On:21 Sep 2008 06:28
Last Modified:08 Nov 2021 22:01

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