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Cell Chromatography: Biocompatible Chromatographic Separation and Interrogation of Microbial Cells

Hazu, M. and Ahmed, A. and Curry, E. and Hornby, D. P. and Gjerde, D. T. (2022) Cell Chromatography: Biocompatible Chromatographic Separation and Interrogation of Microbial Cells. Microbiology Spectrum, 10 (5). ISSN 2165-0497. doi:10.1128/spectrum.02450-22.

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The isolation of pure, single colonies lies at the heart of experimental microbiology. However, a microbial colony typically contains around 1 million cells at all stages of the life cycle. Here, we describe a novel cell chromatography method that facilitates the capture, purification, and interrogation of microbial cell populations from both single and mixed cultures. The method described relies on, but is not limited to, differences in surface charge to separate bacterial strains. The method is fully biocompatible, leading to no significant loss of cell viability. The chromatographic capture of cells, combined with selective elution methods, facilitates a greater level of experimental control over the sample inputs required for downstream high-throughput and high-sensitivity analytical methods. The application of the method for interrogating the antibiotic resistance of bacterial strains and for the separation of bacteria from environmental samples is illustrated.

Item Type:Article
Related URLs:
URLURL TypeDescription ItemDiscussion Paper
Hazu, M.0000-0002-6527-9597
Hornby, D. P.0000-0002-8188-7023
Gjerde, D. T.0000-0002-8315-7083
Issue or Number:5
Record Number:CaltechAUTHORS:20221003-756400000.20
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:117211
Deposited By: Melissa Ray
Deposited On:06 Oct 2022 16:02
Last Modified:01 Nov 2022 22:51

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