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Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators

Zhang, Ruiying and Li, Lei S. and Rao, Bin and Rong, Haoyang and Sun, Min-Yu and Yao, Junjie and Chen, Ruimin and Zhou, Qifa and Mennerick, Steven and Raman, Baranidharan and Wang, Lihong V. (2022) Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators. Journal of Biomedical Optics, 27 (9). Art. No. 096004. ISSN 1083-3668. PMCID PMC9463545. doi:10.1117/1.jbo.27.9.096004. https://resolver.caltech.edu/CaltechAUTHORS:20221017-12147700.17

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Abstract

Significance: Optical imaging of responses in fluorescently labeled neurons has progressed significantly in recent years. However, there is still a need to monitor neural activities at divergent spatial scales and at depths beyond the optical diffusion limit. Aim: To meet these needs, we aim to develop multiscale photoacoustic tomography (PAT) to image neural activities across spatial scales with a genetically encoded calcium indicator GCaMP. Approach: First, using photoacoustic microscopy, we show that depth-resolved GCaMP signals can be monitored in vivo from a fly brain in response to odor stimulation without depth scanning and even with the cuticle intact. In vivo monitoring of GCaMP signals was also demonstrated in mouse brains. Next, using photoacoustic computed tomography, we imaged neural responses of a mouse brain slice at depths beyond the optical diffusion limit. Results: We provide the first unambiguous demonstration that multiscale PAT can be used to record neural activities in transgenic flies and mice with select neurons expressing GCaMP. Conclusions: Our results indicate that the combination of multiscale PAT and fluorescent neural activity indicators provides a methodology for imaging targeted neurons at various scales.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.JBO.27.9.096004DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc9463545/PubMed CentralArticle
ORCID:
AuthorORCID
Zhang, Ruiying0000-0002-0092-0814
Rao, Bin0000-0001-6494-3110
Chen, Ruimin0000-0002-5338-359X
Zhou, Qifa0000-0003-1527-3020
Mennerick, Steven0000-0003-0868-0664
Raman, Baranidharan0000-0002-7866-155X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:The authors thank Prof. James Ballard for a close reading of the manuscript and Terence Wong for experimental help. This work was sponsored in part by the US National Institutes of Health (NIH), grants U01 NS099717 (BRAIN Initiative) to Lihong Wang and 5K99AR062530 (NIH K99/R00) to Bin Rao; an Imaging Sciences Pathway fellowship to Haoyang Rong; and a National Science Foundation (NSF) CAREER grant (#1453022) to Baranidharan Raman.
Funders:
Funding AgencyGrant Number
NIHU01 NS099717
NIH5K99AR062530
NSFIIS-1453022
Issue or Number:9
PubMed Central ID:PMC9463545
DOI:10.1117/1.jbo.27.9.096004
Record Number:CaltechAUTHORS:20221017-12147700.17
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20221017-12147700.17
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:117456
Collection:CaltechAUTHORS
Deposited By: Research Services Depository
Deposited On:20 Oct 2022 16:56
Last Modified:20 Oct 2022 16:56

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