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DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato

Matthew, Louisa and Reyes, Melquiades E. C. and Mann, Christopher W. G. and McDowall, Alasdair W. and Eamens, Andrew L. and Carroll, Bernard J. (2022) DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato. Plants, 11 (19). Art. No. 2545. ISSN 2223-7747. doi:10.3390/plants11192545.

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Most flowering plant species contain at least two copies of the DEFECTIVE EMBRYO AND MERISTEMS (DEM) gene with the encoded DEM proteins lacking homology to proteins of known biochemical function. In tomato (Sl; Solanum lycopersicum), stable mutations in the SlDEM1 locus result in shoot and root meristem defects with the dem1 mutant failing to progress past the cotyledon stage of seedling development. Generation of a Somatic Mutagenesis of DEM1 (SMD) transformant line in tomato allowed for the characterization of SlDEM1 gene function past the seedling stage of vegetative development with SMD plants displaying a range of leaf development abnormalities. Further, the sectored or stable in planta expression of specific regions of the SlDEM1 coding sequence also resulted in the generation of tomato transformants that displayed a range of vegetative development defects, which when considered together with the dem1 mutant seedling and SMD transformant line phenotypic data, allowed for the assignment of SlDEM1 gene function to early embryo development, adaxial epidermis cell development, lateral leaf blade expansion, and mesophyll cell proliferation and differentiation.

Item Type:Article
Related URLs:
URLURL TypeDescription
Mann, Christopher W. G.0000-0001-8431-6317
Eamens, Andrew L.0000-0002-6327-3618
Carroll, Bernard J.0000-0002-1976-8269
Additional Information:This work was supported by Australian Research Council grant CE034821 and internally by The University of Queensland to B.J.C.; L.M. and M.E.C.R. were supported by The University of Queensland PhD Scholarships, and A.L.E. was supported by a University of Queensland funded RSP2 Senior Research Fellowship. Julie Waldron is thanked for technical assistance.
Funding AgencyGrant Number
Australian Research CouncilCE034821
University of QueenslandUNSPECIFIED
Issue or Number:19
Record Number:CaltechAUTHORS:20221021-258279100.6
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:117522
Deposited By: Research Services Depository
Deposited On:27 Oct 2022 22:41
Last Modified:27 Oct 2022 22:41

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