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Raman Scattering Spectroscopy on Strawberry DNA

Kocheril, Philip A. and Jones, Makaela M. and Kubicek-Sutherland, Jessica Z. (2022) Raman Scattering Spectroscopy on Strawberry DNA. Journal of Chemical Education, 99 (10). pp. 3546-3552. ISSN 0021-9584. doi:10.1021/acs.jchemed.2c00687. https://resolver.caltech.edu/CaltechAUTHORS:20221024-123751900.12

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Abstract

Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from strawberries with a soap solution and precipitated in isopropanol, two major Raman peaks with shifts of 740 and 2970 cm⁻¹ were observed, consistent with previously published DNA Raman spectra. In this general article, we discuss the potential implementation of this work in an upper-level undergraduate laboratory, which would allow for hands-on exposure to both spectroscopic instrumental design and biochemical sample complexity.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.jchemed.2c00687DOIArticle
ORCID:
AuthorORCID
Kocheril, Philip A.0000-0002-1265-5931
Jones, Makaela M.0000-0002-7072-3904
Kubicek-Sutherland, Jessica Z.0000-0001-6215-4871
Additional Information:The graphical abstract and Figure 1 were created with BioRender.com. This work was funded by the Los Alamos National Laboratory Strategic Investment Initiative of the Richard P. Feynman Center for Innovation. This work was performed at the Los Alamos National Laboratory, which is operated by Triad National Security, LLC, for the National Nuclear Security Administration of the U.S. Department of Energy (contract 89233218CNA000001).
Funders:
Funding AgencyGrant Number
Los Alamos National LaboratoryUNSPECIFIED
Department of Energy (DOE)89233218CNA000001
Issue or Number:10
DOI:10.1021/acs.jchemed.2c00687
Record Number:CaltechAUTHORS:20221024-123751900.12
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20221024-123751900.12
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:117543
Collection:CaltechAUTHORS
Deposited By: Research Services Depository
Deposited On:28 Oct 2022 22:56
Last Modified:01 Nov 2022 17:24

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