Ravanfar, Raheleh and Sheng, Yuling and Gray, Harry B. and Winkler, Jay R. (2023) Tryptophan-96 in cytochrome P450 BM3 plays a key role in enzyme survival. FEBS Letters, 597 (1). pp. 59-64. ISSN 0014-5793. doi:10.1002/1873-3468.14514. https://resolver.caltech.edu/CaltechAUTHORS:20221107-999291600.27
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20221107-999291600.27
Abstract
Flavocytochrome P450 from Bacillus megaterium (P450_(BM3)) is a natural fusion protein containing reductase and hame domains. In the presence of NADPH and dioxygen the enzyme catalyses the hydroxylation of long-chain fatty acids. Analysis of the P450BM3 structure reveals chains of closely spaced tryptophan and tyrosine residues that might serve as pathways for high-potential oxidizing equivalents to escape from the hame active site when substrate oxidation is not possible. Our investigations of the total number of enzyme turnovers before deactivation have revealed that replacement of selected tryptophan and tyrosine residues with redox inactive groups leads to a twofold reduction in enzyme survival time. Tryptophan-96 is critical for prolonging enzyme activity, suggesting a key protective role for this residue.
| Item Type: | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||
| ORCID: |
| ||||||||
| Additional Information: | This research was supported by the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number R01DK019038. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Additional support was provided by the Arnold and Mabel Beckman Foundation. | ||||||||
| Funders: |
| ||||||||
| Issue or Number: | 1 | ||||||||
| DOI: | 10.1002/1873-3468.14514 | ||||||||
| Record Number: | CaltechAUTHORS:20221107-999291600.27 | ||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20221107-999291600.27 | ||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||
| ID Code: | 117755 | ||||||||
| Collection: | CaltechAUTHORS | ||||||||
| Deposited By: | Research Services Depository | ||||||||
| Deposited On: | 18 Nov 2022 21:26 | ||||||||
| Last Modified: | 25 Jan 2023 20:42 |
Repository Staff Only: item control page
