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Geometric effects in gas vesicle buckling under ultrasound

Salahshoor, Hossein and Yao, Yuxing and Dutka, Przemysław and Nyström, Nivin N. and Jin, Zhiyang and Min, Ellen and Malounda, Dina and Jensen, Grant J. and Ortiz, Michael and Shapiro, Mikhail G. (2022) Geometric effects in gas vesicle buckling under ultrasound. Biophysical Journal, 121 (21). pp. 4221-4228. ISSN 0006-3495. PMCID PMC9674984. doi:10.1016/j.bpj.2022.09.004.

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Acoustic reporter genes based on gas vesicles (GVs) have enabled the use of ultrasound to noninvasively visualize cellular function in vivo. The specific detection of GV signals relative to background acoustic scattering in tissues is facilitated by nonlinear ultrasound imaging techniques taking advantage of the sonomechanical buckling of GVs. However, the effect of geometry on the buckling behavior of GVs under exposure to ultrasound has not been studied. To understand such geometric effects, we developed computational models of GVs of various lengths and diameters and used finite element simulations to predict their threshold buckling pressures and postbuckling deformations. We demonstrated that the GV diameter has an inverse cubic relation to the threshold buckling pressure, whereas length has no substantial effect. To complement these simulations, we experimentally probed the effect of geometry on the mechanical properties of GVs and the corresponding nonlinear ultrasound signals. The results of these experiments corroborate our computational predictions. This study provides fundamental insights into how geometry affects the sonomechanical properties of GVs, which, in turn, can inform further engineering of these nanostructures for high-contrast, nonlinear ultrasound imaging.

Item Type:Article
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URLURL TypeDescription ItemDiscussion Paper
Salahshoor, Hossein0000-0002-7264-7650
Yao, Yuxing0000-0003-0337-6372
Dutka, Przemysław0000-0003-3819-1618
Nyström, Nivin N.0000-0001-6288-6060
Jin, Zhiyang0000-0002-4411-6991
Malounda, Dina0000-0001-7086-9877
Jensen, Grant J.0000-0003-1556-4864
Ortiz, Michael0000-0001-5877-4824
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:The authors are grateful to Ngozi A. Eze for the helpful editorial comments. The authors are also grateful to Dr. Di Wu for insightful discussions and input. This research was supported by the National Institutes of Health grant R01-EB018975. Related research in the Shapiro lab is supported by the Packard Foundation, The Pew Charitable Trusts, and the Chan Zuckerberg Initiative. Cryo-EM was performed at the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. M.G.S. is an investigator of the Howard Hughes Medical Institute (HHMI). This article is subject to HHMI’s Open Access to Publications policy. HHMI Investigators have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication.
Funding AgencyGrant Number
David and Lucile Packard FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Pew Charitable TrustsUNSPECIFIED
Chan Zuckerberg InitiativeUNSPECIFIED
Issue or Number:21
PubMed Central ID:PMC9674984
Record Number:CaltechAUTHORS:20221128-494241100.5
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:118051
Deposited By: Research Services Depository
Deposited On:07 Dec 2022 16:53
Last Modified:07 Dec 2022 16:53

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