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The application of Fresnel zone plate based projection in optofluidic microscopy

Wu, Jigang and Cui, Xiquan and Lee, Lap Man and Yang, Changhuei (2008) The application of Fresnel zone plate based projection in optofluidic microscopy. Optics Express, 16 (20). pp. 15595-15602. ISSN 1094-4087. PMCID PMC2688452.

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Optofluidic microscopy (OFM) is a novel technique for low-cost, high-resolution on-chip microscopy imaging. In this paper we report the use of the Fresnel zone plate (FZP) based projection in OFM as a cost-effective and compact means for projecting the transmission through an OFM's aperture array onto a sensor grid. We demonstrate this approach by employing a FZP (diameter = 255 µm, focal length = 800 µm) that has been patterned onto a glass slide to project the transmission from an array of apertures (diameter = 1 µm, separation = 10 µm) onto a CMOS sensor. We are able to resolve the contributions from 44 apertures on the sensor under the illumination from a HeNe laser (wavelength = 633 nm). The imaging quality of the FZP determines the effective field-of-view (related to the number of resolvable transmissions from apertures) but not the image resolution of such an OFM system -- a key distinction from conventional microscope systems. We demonstrate the capability of the integrated system by flowing the protist Euglena gracilis across the aperture array microfluidically and performing OFM imaging of the samples.

Item Type:Article
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Additional Information:© 2008 Optical Society of America. Original Manuscript: July 17, 2008. Manuscript Accepted: September 11, 2008. Revised Manuscript: September 6, 2008. Published: September 18, 2008. The authors would like to acknowledge Dr. Xin Heng for helpful discussions. This project is supported by the NSF career award BES-0547657.
Group:Kavli Nanoscience Institute
Funding AgencyGrant Number
Subject Keywords:optofluidics; lab-on-a-chip; diffractive optics; imaging systems; apertures; integrated optics
Issue or Number:20
PubMed Central ID:PMC2688452
Record Number:CaltechAUTHORS:WUJoe08
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Official Citation:J. Wu, X. Cui, L. M. Lee, and C. Yang, "The application of Fresnel zone plate based projection in optofluidic microscopy," Opt. Express 16, 15595-15602 (2008)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11817
Deposited By: Archive Administrator
Deposited On:02 Oct 2008 03:30
Last Modified:03 Oct 2019 00:22

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