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Combined SHOOT MERISTEMLESS and WUSCHEL trigger ectopic organogenesis in Arabidopsis

Gallois, Jean-Luc and Woodward, Claire and Reddy, G. Venugopala and Sablowski, Robert (2002) Combined SHOOT MERISTEMLESS and WUSCHEL trigger ectopic organogenesis in Arabidopsis. Development, 129 (13). pp. 3207-3217. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:GALdev02

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Abstract

Almost all aerial parts of plants are continuously generated at the shoot apical meristem (SAM). To maintain a steady pool of undifferentiated cells in the SAM while continuously generating new organs, it is necessary to balance the rate of cell division with the rate of entrance into differentiation pathways. In the Arabidopsis meristem, SHOOT MERISTEMLESS (STM) and WUSCHEL (WUS) are necessary to keep cells undifferentiated and dividing. Here, we tested whether ectopic STM and WUS functions are sufficient to revert differentiation and activate cell division in differentiating tissues. Ectopic STM and WUS functions interacted non-additively and activated a subset of meristem functions, including cell division, CLAVATA1 expression and organogenesis, but not correct phyllotaxy or meristem self-maintenance. Our results suggest that WUS produces a non-cell autonomous signal that activates cell division in combination with STM and that combined WUS/STM functions can initiate the progression from stem cells to organ initiation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/129/13/3207PublisherArticle
Additional Information:© 2002 The Company of Biologists Limited. Accepted 9 April 2002. We are grateful to Jan Traas (INRA, Versailles) for the KNAT2::uidA line, to John Doonan (John Innes Centre) for cycD3::uidA, Peter Doerner (ICMB, Edimburgh) for cycB1::uidA, Pat Springer (University of California, Riverside) for LOB, Leslie Sieburth (Ohio State University) for hsp18.2::Cre, Jim Haseloff (University of Cambridge) for mGFP5-ER, Jeff Long (Caltech, Pasadena) for stm11, Kevin Roberg (Caltech, Pasadena) for help with creating the CLV1::GFP reporter, Grant Calder and Kim Findlay (John Innes Centre) for assistance with microscopy, and to Desmond Bradley, Cathie Martin and Carla Schommer for critical reading of the manuscript. J.L.G. was funded by the European Union (Framework V – Regulatory Gene Initiative in Arabidopsis, QLG2-CT-199900876); C.W. received a BBSRC/Syngenta CASE studentship; G.V.R. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Work in the Sablowski lab is funded by BBSRC, European Union-FPV and Syngenta.
Funders:
Funding AgencyGrant Number
Eurpoean UnionQLG2-CT-199900876
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
SyngentaUNSPECIFIED
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
Subject Keywords:Arabidopsis thaliana, Meristem establishment, WUS, STM
Issue or Number:13
Record Number:CaltechAUTHORS:GALdev02
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GALdev02
Official Citation:Combined SHOOT MERISTEMLESS and WUSCHEL trigger ectopic organogenesis in Arabidopsis Jean-Luc Gallois, Claire Woodward, G. Venugopala Reddy, Robert Sablowski Development 2002 129: 3207-3217
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11826
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Oct 2008 20:10
Last Modified:03 Oct 2019 00:22

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