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A Cristae-Like Microcompartment in Desulfobacterota

McGlynn, Shawn Erin and Perkins, Guy and Sim, Min Sub and Mackey, Mason and Deerinck, Thomas J. and Thor, Andrea and Phan, Sebastien and Ballard, Daniel and Ellisman, Mark H. and Orphan, Victoria J. (2022) A Cristae-Like Microcompartment in Desulfobacterota. mBio, 13 (6). Art. No. 01613-22. ISSN 2150-7511. PMCID PMC9764997. doi:10.1128/mbio.01613-22.

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Some Alphaproteobacteria contain intracytoplasmic membranes (ICMs) and proteins homologous to those responsible for the mitochondrial cristae, an observation which has given rise to the hypothesis that the Alphaproteobacteria endosymbiont had already evolved cristae-like structures and functions. However, our knowledge of microbial fine structure is still limited, leaving open the possibility of structurally homologous ICMs outside the Alphaproteobacteria. Here, we report on the detailed characterization of lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota that form syntrophic partnerships with anaerobic methane-oxidizing (ANME) archaea. These structures are junction-bound to the cytoplasmic membrane and resemble the form seen in the lamellar cristae of opisthokont mitochondria. Extending these observations, we also characterized similar structures in Desulfovibrio carbinolicus, a close relative of the magnetotactic D. magneticus, which does not contain magnetosomes. Despite a remarkable structural similarity, the key proteins involved in cristae formation have not yet been identified in Desulfobacterota, suggesting that an analogous, but not a homologous, protein organization system developed during the evolution of some members of Desulfobacterota.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
McGlynn, Shawn Erin0000-0002-8199-7011
Perkins, Guy0000-0002-1834-6646
Sim, Min Sub0000-0002-3491-9002
Phan, Sebastien0000-0003-4261-6973
Ellisman, Mark H.0000-0001-8893-8455
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:This study is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, under Award Number DE-SC0020373 to (V.J.O. and M.H.E.), by support from the Gordon and Betty Moore Foundation Symbiosis Initiative (V.J.O.), by support from the Simons Foundation Project on the Origin of the Eukaryotic Cell (M.H.E.), and by National Institutes of Health (NIH) grant P41 GM103412 to M.H.E. S.E.M. was supported in part by a MEXT KAKENHI Grant-in-Aid for challenging exploratory research (grant award number 15K14608) and the Research Foundation for Opto-Science and Technology. This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. We declare that there are no competing interests.
Funding AgencyGrant Number
Department of Energy (DOE)DE-SC0020373
Gordon and Betty Moore FoundationUNSPECIFIED
Simons FoundationUNSPECIFIED
NIHP41 GM103412
Ministry of Education, Culture, Sports, Science and Technology (MEXT)15K14608
Research Foundation for Opto-Science and TechnologyUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC9764997
Record Number:CaltechAUTHORS:20221212-796746500.36
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:118321
Deposited By: Research Services Depository
Deposited On:14 Jan 2023 21:15
Last Modified:14 Jan 2023 21:15

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