A Caltech Library Service

Behind the developing brains and beating hearts of stem cell-derived embryo models

Amadei, Gianluca and Glover, David M. (2023) Behind the developing brains and beating hearts of stem cell-derived embryo models. Open Biology, 13 (1). Art. No. rsob.220325. ISSN 2046-2441. PMCID PMC9833437. doi:10.1098/rsob.220325.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


Studies over the past decade have shown how stem cells representing embryonic and extra-embryonic tissues of the mouse can self-assemble in the culture dish to recapitulate an astonishing part of early embryonic development. A systematic analysis has demonstrated how pluripotent embryonic stem cells can be induced to behave like the implanting epiblast; how they can interact with trophectoderm stem cells to form a patterned structure resembling the implanting embryo prior to gastrulation; and how the third stem cell type—extra-embryonic endoderm cells—can be incorporated to generate structures that undergo the cell movements and gene expression patterns of gastrulation. Moreover, such stem cell-derived embryo models can proceed to neurulation and establish progenitors for all parts of the brain and neural tube, somites, beating heart structures and gut tube. They develop within extra-embryonic yolk sacs that initiate haematopoiesis. Here we trace this journey of discovery.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Amadei, Gianluca0000-0001-5405-968X
Glover, David M.0000-0003-0956-0103
Additional Information:D.M.G. has been priviledged to be a fly-person on the wall throughout these studies, occasionally falling into the soup but managing to keep afloat. In the course of this work, we are grateful for many discussions with Magdalena Zernicka-Goetz, Ivan Bedzhov, Sarah Harrison, Marta Shahbazi, Berna Sozen, Andy Cox, Charlotte Handford and Kasey Lau, among many other members of the Zernicka-Goetz lab. This article does not aim to be a comprehensive review of artificial embryos and we apologize for not being able to review or cite all publications in the field. We received no funding for this study.
Issue or Number:1
PubMed Central ID:PMC9833437
Record Number:CaltechAUTHORS:20230124-9343400.1
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:118911
Deposited By: Research Services Depository
Deposited On:01 Feb 2023 18:23
Last Modified:01 Feb 2023 18:24

Repository Staff Only: item control page