A Caltech Library Service

Optical-resolution photoacoustic microscopy with a needle-shaped beam

Cao, Rui and Zhao, Jingjing and Li, Lei and Du, Lin and Zhang, Yide and Luo, Yilin and Jiang, Laiming and Davis, Samuel and Zhou, Qifa and de la Zerda, Adam and Wang, Lihong V. (2023) Optical-resolution photoacoustic microscopy with a needle-shaped beam. Nature Photonics, 17 (1). pp. 89-95. ISSN 1749-4885. doi:10.1038/s41566-022-01112-w.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


Optical-resolution photoacoustic microscopy can visualize wavelength-dependent optical absorption at the cellular level. However, this technique suffers from a limited depth of field due to the tight focus of the optical excitation beam, making it challenging to acquire high-resolution images of samples with uneven surfaces or high-quality volumetric images without z scanning. To overcome this limitation, we propose needle-shaped beam photoacoustic microscopy, which can extend the depth of field to around a 28-fold Rayleigh length via customized diffractive optical elements. These diffractive optical elements generate a needle-shaped beam with a well-maintained beam diameter, a uniform axial intensity distribution and negligible sidelobes. The advantage of using needle-shaped beam photoacoustic microscopy is demonstrated via both histology-like imaging of fresh slide-free organs using a 266 nm laser and in vivo mouse-brain vasculature imaging using a 532 nm laser. This approach provides new perspectives for slide-free intraoperative pathological imaging and in vivo organ-level imaging.

Item Type:Article
Related URLs:
URLURL TypeDescription ReadCube access InCaltech News
Cao, Rui0000-0003-4444-7528
Li, Lei0000-0001-6164-2646
Du, Lin0000-0002-6111-4947
Zhang, Yide0000-0002-9463-3970
Luo, Yilin0000-0002-4611-3049
Jiang, Laiming0000-0002-8658-3168
Davis, Samuel0000-0002-0871-8880
Zhou, Qifa0000-0003-1527-3020
de la Zerda, Adam0000-0002-5617-0194
Wang, Lihong V.0000-0001-9783-4383
Additional Information:L.V.W. was sponsored by the United States National Institutes of Health grants R01 EB028277, U01 NS099717 (BRAIN Initiative) and R35 CA220436 (Outstanding Investigator Award). A.d.l.Z. was supported by the National Institutes of Health grants DP50D012179 and K23CA211793, the United States National Science Foundation (NSF 1438340) and the United States Air Force (FA9550–15–1–0007).
Group:Tianqiao and Chrissy Chen Institute for Neuroscience
Funding AgencyGrant Number
NIHR01 EB028277
NIHU01 NS099717
NIHR35 CA220436
Air Force Office of Scientific Research (AFOSR)FA9550-15-1-0007
Issue or Number:1
Record Number:CaltechAUTHORS:20230125-514949300.27
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:118946
Deposited By: Research Services Depository
Deposited On:31 Jan 2023 17:01
Last Modified:11 Apr 2023 15:35

Repository Staff Only: item control page