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Control of protein activity by photoinduced spin polarized charge reorganization

Ghosh, Shirsendu and Banerjee-Ghosh, Koyel and Levy, Dorit and Scheerer, David and Riven, Inbal and Shin, Jieun and Gray, Harry B. and Naaman, Ron and Haran, Gilad (2022) Control of protein activity by photoinduced spin polarized charge reorganization. Proceedings of the National Academy of Sciences, 119 (35). Art. No. e2204735119. ISSN 0027-8424. PMCID PMC9436351.

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Considerable electric fields are present within living cells, and the role of bioelectricity has been well established at the organismal level. Yet much remains to be learned about electric-field effects on protein function. Here, we use phototriggered charge injection from a site-specifically attached ruthenium photosensitizer to directly demonstrate the effect of dynamic charge redistribution within a protein. We find that binding of an antibody to phosphoglycerate kinase (PGK) is increased twofold under illumination. Remarkably, illumination is found to suppress the enzymatic activity of PGK by a factor as large as three. These responses are sensitive to the photosensitizer position on the protein. Surprisingly, left (but not right) circularly polarized light elicits these responses, indicating that the electrons involved in the observed dynamics are spin polarized, due to spin filtration by protein chiral structures. Our results directly establish the contribution of electrical polarization as an allosteric signal within proteins. Future experiments with phototriggered charge injection will allow delineation of charge rearrangement pathways within proteins and will further depict their effects on protein function.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle ItemDiscussion Paper
Ghosh, Shirsendu0000-0002-0820-9349
Banerjee-Ghosh, Koyel0000-0002-1376-5198
Scheerer, David0000-0002-6641-6629
Riven, Inbal0000-0002-8268-1377
Shin, Jieun0000-0003-3817-6282
Gray, Harry B.0000-0002-7937-7876
Naaman, Ron0000-0003-1910-366X
Haran, Gilad0000-0003-1837-9779
Additional Information:© 2022 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY). This work was partially supported by a grant to G.H. from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 742637, SMALLOSTERY), a grant from the Israel Science Foundation (no. 1250/19) and a grant from the Weizmann SABRA-Yeda-Sela-WRC program. G.H. holds the Hilda Pomeraniec Memorial Professorial Chair. R.N. acknowledges partial support from the MINERVA Foundation, from the Israel Ministry of Science and Technology, and from the AFOSR (FA9550-21-1-0418). Work at Caltech was supported by the United States NIH (R01 DK019038 to H.B.G.). Data, Materials, and Software Availability. All study data are included in the article and/or supporting information.
Funding AgencyGrant Number
European Research Council (ERC)742637
Israel Science Foundation1250/19
Weizmann Institute of ScienceUNSPECIFIED
Ministry of Science and Technology (Israel)UNSPECIFIED
Air Force Office of Scientific Research (AFOSR)FA9550-21-1-0418
Issue or Number:35
PubMed Central ID:PMC9436351
Record Number:CaltechAUTHORS:20230210-192713382
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:119198
Deposited By: Research Services Depository
Deposited On:22 Mar 2023 16:43
Last Modified:22 Mar 2023 16:43

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