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Cell monitoring with optical coherence tomography

Brehove, Matthew and Rogers, Claude and Menon, Rudra and Minor, Paul and Allington, James and Lam, Annie and Vielmetter, Jost and Menon, Naresh (2023) Cell monitoring with optical coherence tomography. Cytotherapy, 25 (2). pp. 120-124. ISSN 1465-3249. doi:10.1016/j.jcyt.2022.09.008.

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Background aims: We evaluated a commercially available instrument, OCTiCell (, for monitoring cell growth in suspended agitated bioreactors based on optical coherence tomography. OCTiCell is an in-line, completely non-invasive instrument that can operate on any suspended-cell bioreactor with a window or transparent wall. In traditional optical coherence tomography, the imaging beam is rastered over the sample to form a three-dimensional image. OCTiCell, instead uses a fixed imaging beam and takes advantage of the motion of the media to move the cells across the interrogating optical beam. Results: We found strong correlations between the non-invasive, non-contact, reagent-free OCTiCell measurements of cell concentration and viability and those obtained from the automated cell counter, and the XTT viability assay, which is a colorimetric assay for quantifying metabolic activity. Conclusions: This novel cell monitoring method can adapt to different bioreactor form factors and could reduce the labor cost and contamination risks associated with cell growth monitoring.

Item Type:Article
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Vielmetter, Jost0000-0002-4314-7163
Menon, Naresh0000-0003-1359-6280
Additional Information:This work was funded by the Defense Health Agency Contract No. W81XWH-19-C-0081. ChromoLogic LLC staff adheres strictly to Ethical Scientific Conduct guidelines documented by the company in their Employee Agreement, and all workers adhere to guidelines established by the National Institutes of Health.
Funding AgencyGrant Number
Defense Health AgencyW81XWH-19-C-0081
Issue or Number:2
Record Number:CaltechAUTHORS:20230213-466109600.13
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:119242
Deposited By: Research Services Depository
Deposited On:24 Mar 2023 19:18
Last Modified:24 Mar 2023 19:18

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