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Mob4 is essential for spermatogenesis in Drosophila melanogaster

Santos, Inês B. and Wainman, Alan and Garrido-Maraver, Juan and Pires, Vanessa and Riparbelli, Maria Giovanna and Kovács, Levente and Callaini, Giuliano and Glover, David M. and Tavares, Álvaro A. (2023) Mob4 is essential for spermatogenesis in Drosophila melanogaster. . (Unpublished)

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Gamete formation is essential for sexual reproduction in metazoans. In males, spermatogenesis gives rise to interconnected spermatids that have to differentiate and individualize into mature sperm. InDrosophila melanogaster, individualization of spermatids requires the formation of individualization complexes that synchronously move along the sperm bundles. Here, we show that Mob4, a member of the Mps-one binder family, is essential for male fertility but has no detectable role on female fertility. We describe the function of Mob4 during spermatid individualization, showing that Mob4 is required for proper axonemal structure and that the loss of Mob4 leads to male sterility associated with defective spermatid individualization and absence of mature sperm in the seminal vesicles. Transmission electron micrographs of Mob4ᴿᴺᴬᶦ developing spermatids revealed defects in axoneme structure and abnormal mitochondria biogenesis. Importantly, we find that male fertility is impaired upon depletion of other STRIPAK components, suggesting that Mob4 acts through STRIPAK to support spermiogenesis. As we show that expression of the human Mob4 gene effectively rescues all phenotypes of Mob4 downregulation, the gene is not only evolutionary but also functionally conserved. We propose that Mob4 plays a role in regulating the microtubule- and actin-cytoskeleton during spermatogenesis. This study advances our understanding of male infertility by uncovering Mob4 as a novel gene required for sperm individualization.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Wainman, Alan0000-0002-6292-4183
Garrido-Maraver, Juan0000-0003-4534-4109
Pires, Vanessa0000-0002-1514-685X
Riparbelli, Maria Giovanna0000-0003-2084-019X
Kovács, Levente0000-0002-3226-3740
Callaini, Giuliano0000-0003-2252-0309
Glover, David M.0000-0003-0956-0103
Tavares, Álvaro A.0000-0002-7137-0944
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Work funded by Algarve 2020 Program, grant number ALG-01-0145-FEDER-030014, and co-financed by FEDER Funds through the Operational Program for Competitiveness Factors—COMPETE 2020 and by National Funds through FCT—Foundation for Science and Technology under the Project PTDC/BIA377 CEL/30014/2017. Inês B. Santos funded by FCT fellowship SFRH/BD/141734/2018. Giulliano Callaini funded by Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR 2020CLZ5XW). Drosophila stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. We also thank Flybase (Gramates LS et al., 2022) and FlyAtlas (Krause SA et al., 2022) for providing database information. The authors have declared no competing interest.
Funding AgencyGrant Number
University of AlgarveALG-01-0145-FEDER-030014
Fondo Europeo de Desarrollo Regional (FEDER)UNSPECIFIED
Fundação para a Ciência e a Tecnologia (FCT)PTDC/BIA377 CEL/30014/2017
Fundação para a Ciência e a Tecnologia (FCT)SFRH/BD/141734/2018
Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR)2020CLZ5XW
Record Number:CaltechAUTHORS:20230316-182273000.24
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:120141
Deposited By: George Porter
Deposited On:21 Mar 2023 22:49
Last Modified:21 Mar 2023 22:49

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