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Cytokinins regulate spatially-specific ethylene production to control root growth in Arabidopsis

Yamoune, Amel and Zdarska, Marketa and Depaepe, Thomas and Korytarova, Anna and Skalak, Jan and Berendzen, Kenneth Wayne and Mira-Rodado, Virtudes and Tarr, Paul and Spackova, Eliska and Badurova, Lucia and Parizkova, Barbora and Franczyk, Abigail and Kovacova, Ingrid and Pernisova, Marketa and Novak, Ondrej and Meyerowitz, Elliot and Harter, Klaus and Van Der Straeten, Dominique and Hejátko, Jan (2023) Cytokinins regulate spatially-specific ethylene production to control root growth in Arabidopsis. . (Unpublished)

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The two principal growth regulators cytokinins and ethylene are known to interact in the regulation of plant growth. However, information about underlying molecular mechanism and positional specificity of the cytokinin/ethylene crosstalk in root growth control is scarce. We have identified spatial specificity of cytokinin-regulated root elongation and root apical meristem (RAM) size, both of which we demonstrate to be ethylene biosynthesis-dependent. Upregulation of the cytokinin biosynthetic gene ISOPENTENYLTRANSFERASE(IPT) in proximal and peripheral tissues leads to both root and RAM shortening. In contrast, IPTactivation in distal and inner tissues reduces RAM size while leaving the root length comparable to mock-treated controls. We show that cytokinins regulate two steps specific to ethylene biosynthesis, the production of ACC by ACC SYNTHASEs (ACSs), and its conversion to ethylene by ACC OXIDASEs (ACOs). We describe cytokinin- and ethylene-specific regulation controlling the activity of ACSs and ACOs that are spatially discrete along both proximo/distal and radial root axes. Using direct ethylene measurements, we identifyACO2, ACO3andACO4as being responsible for ethylene biosynthesis and the ethylene-regulated root and RAM shortening in cytokinin-treated Arabidopsis. Finally, we describe the tight cooperation between cytokinin and ethylene signaling in cytokinin-induced, ethylene-regulated control ofACO4due to the direct interaction between ARABIDOPSIS RESPONSE REGULATOR 2 (ARR2), a member of the multistep phosphorelay cascade and the C-terminal portion of ETHYLENE INSENSITIVE 2 (EIN2-C), a key regulator of canonical ethylene signaling.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Yamoune, Amel0000-0002-5112-878X
Zdarska, Marketa0000-0002-5830-3122
Depaepe, Thomas0000-0003-2656-3437
Skalak, Jan0000-0002-0784-499X
Berendzen, Kenneth Wayne0000-0001-9870-6164
Mira-Rodado, Virtudes0000-0003-0492-7885
Tarr, Paul0000-0003-2977-3683
Parizkova, Barbora0000-0002-8125-2271
Pernisova, Marketa0000-0002-5803-2879
Novak, Ondrej0000-0003-3452-0154
Meyerowitz, Elliot0000-0003-4798-5153
Harter, Klaus0000-0002-2150-6970
Van Der Straeten, Dominique0000-0002-7755-1420
Hejátko, Jan0000-0002-2622-6046
Alternate Title:Cytokinin‐induced ethylene in root growth
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. We are very grateful to prof. Li‐Jia Qu for providing us with seeds of ethylene‐free lines (Li et al., 2022). We acknowledge the core facility CELLIM of CEITEC supported by the MEYS CR (LM2018129 Czech‐BioImaging). Core Facility Plants Sciences of CEITEC MU is gratefully acknowledged for obtaining scientific data presented in this paper. This article is subject to HHMI’s Open Access to Publications policy. HHMI lab heads have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author‐accepted manuscript of this article can be made freely available under a CC BY 4.0 license immediately upon publication. The authors have declared no competing interest.
Funding AgencyGrant Number
Ministry of Education, Youth and Sports (Czech Republic)LM2018129
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20230316-182598000.45
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:120158
Deposited By: George Porter
Deposited On:18 Mar 2023 01:57
Last Modified:18 Mar 2023 01:57

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