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Transformation of shoots into roots in Arabidopsis embryos mutant at the TOPLESS locus

Long, Jeff A. and Woody, Scott and Poethig, Scott and Meyerowitz, Elliot M. and Barton, M. Kathryn (2002) Transformation of shoots into roots in Arabidopsis embryos mutant at the TOPLESS locus. Development, 129 (12). pp. 2797-2806. ISSN 0950-1991.

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We describe a novel phenotype in Arabidopsis embryos homozygous for the temperature-sensitive topless-1 mutation. This mutation causes the transformation of the shoot pole into a root. Developing topless embryos fail to express markers for the shoot apical meristem (SHOOT MERISTEMLESS and UNUSUAL FLORAL ORGANS) and the hypocotyl (KNAT1). By contrast, the pattern of expression of root markers is either duplicated (LENNY, J1092) or expanded (SCARECROW). Shifts of developing topless embryos between permissive and restrictive temperatures show that apical fates (cotyledons plus shoot apical meristem) can be transformed to basal fates (root) as late as transition stage. As the apical pole of transition stage embryos shows both morphological and molecular characteristics of shoot development, this demonstrates that the topless 1 mutation is capable of causing structures specified as shoot to be respecified as root. Finally, our experiments fail to show a clear link between auxin signal transduction and topless-1 mutant activity: the development of the apical root in topless mutant individuals is not dependent on the activity of the predicted auxin response factor MONOPTEROS nor is the expression of DR5, a proposed ‘auxin maximum reporter’, expanded in the apical domain of topless embryos.

Item Type:Article
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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2002 The Company of Biologists Limited. Accepted 19 March 2002. This is paper number 3525 from the Laboratory of Genetics. We thank members of the Barton laboratory and Donna Fernandez for helpful comments on the manuscript. We also acknowledge the support and generosity of Venkatesan Sundaresan in whose laboratory the work on the LENNY marker was initiated. The work in this manuscript was supported by NSF grant #IBN-9973579 to M.K.B., a Helen Hay Whitney Postdoctoral Fellowship to J.L. and NSF grant number MCB-9603821 to E.M. [N.B. PDF carries pagingation, and cumulative pagination in the header, 129, 2297-2306. Actual pagination is 129(12):2797-2806.]
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Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:Arabidopsis, TOPLESS, Shoot transformation, Embryogenesis, Polarity
Issue or Number:12
Record Number:CaltechAUTHORS:LONdev02
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Official Citation:Transformation of shoots into roots in Arabidopsis embryos mutant at the TOPLESS locus Jeff A. Long, Scott Woody, Scott Poethig, Elliot M. Meyerowitz, M. Kathryn Barton Development 2002 129: 2797-2806
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12068
Deposited By: Archive Administrator
Deposited On:21 Oct 2008 22:25
Last Modified:03 Oct 2019 00:25

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