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Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways

Gu, Jennifer L. and Müller, Stefan and Mancino, Valeria and Offermanns, Stefan and Simon, Melvin I. (2002) Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways. Proceedings of the National Academy of Sciences of the United States of America, 99 (14). pp. 9352-9357. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:JUGpnas02

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Abstract

The G(12) subfamily of heterotrimeric G-proteins consists of two members, G(12) and G(13). Gene-targeting studies have revealed a role for G(13) in blood vessel development. Mice lacking the a subunit of G(13) die around embryonic day 10 as the result of an angiogenic defect. On the other hand, the physiological role of G(12) is still unclear. To address this issue, we generated Galpha(12)-deficient mice. In contrast to the Galpha(13)-deficient mice, Galpha(12)-deficient mice are viable, fertile, and do not show apparent abnormalities. However, Galpha(12) does not seem to be entirely redundant, because in the offspring generated from Galpha(12)+/-Galpha(13) intercrosses, at least one intact Galpha(12) allele is required for the survival of animals with only one Galpha(13) allele. In addition, Galpha(12) and Galpha(13) showed a difference in mediating cell migratory response to lysophosphatidic acid in embryonic fibroblast cells. Furthermore, mice lacking both Galpha(12) and Galpha(q) die in utero at about embryonic day 13. These data indicate that the Galpha(12)-mediated signaling pathway functionally interacts not only with the Galpha(13)- but also with the Galpha(q/11)-mediated signaling systems.


Item Type:Article
Additional Information:Copyright © 2002 by the National Academy of Sciences. Contributed by Melvin I. Simon, May 15, 2002. Published online before print June 20, 2002, 10.1073/pnas.102291599 This work is supported in part by National Institutes of Health Grant AG12288 awarded (to M.I.S.). J.L.G. is the recipient of National Research Service Award postdoctoral award 5 F32 HL10496-02. S.M. is the recipient of Deutsche Forschungsgemeinschaft Grant MU 1352/1-1. J.L.G. and S.M. contributed equally to this work
Subject Keywords:G-PROTEIN G(13), HETEROTRIMERIC G-PROTEINS, GTPASE-DEFICIENT MUTANT, SERUM RESPONSE ELEMENT, FOCAL ADHESION KINASE, ALPHA-SUBUNIT, LYSOPHOSPHATIDIC ACID, TRANSFORMING ACTIVITY, PLATELET ACTIVATION, G13 PROTEINS
Issue or Number:14
Record Number:CaltechAUTHORS:JUGpnas02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:JUGpnas02
Alternative URL:http://dx.doi.org/10.1073/pnas.102291599
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1210
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Jan 2006
Last Modified:14 Nov 2014 19:18

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